P2RY1 Protein

Catalog No. ABIN7538493

This Synthetic Nanodisc P2RY1 protein is expressed in Mammalian Cells.

Quick Overview for P2RY1 Protein (ABIN7538493)

Target: P2RY1 (Purinergic Receptor P2Y, G-Protein Coupled, 1 (P2RY1))

Protein Type: Synthetic Nanodisc

Origin: Human

Source: Mammalian Cells

Product Details

Purpose: Human P2RY1 full length protein-synthetic nanodisc

Characteristics: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Application Details

Comment:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: P2RY1 (Purinergic Receptor P2Y, G-Protein Coupled, 1 (P2RY1))

Alternative Name: P2RY1

Background: The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor functions as a receptor for extracellular ATP and ADP. In platelets binding to ADP leads to mobilization of intracellular calcium ions via activation of phospholipase C, a change in platelet shape, and probably to platelet aggregation. [provided by RefSeq, Jul 2008]

Molecular Weight: The human full length P2RY1 protein has a MW of 42.1kDa

UniProt: P47900

Pathways: Regulation of Carbohydrate Metabolic Process, Feeding Behaviour