P2RY10 Protein
Quick Overview for P2RY10 Protein (ABIN7538494)
Target
See all P2RY10 ProteinsProtein Type
Origin
Source
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Purpose
- Human P2Y10 full length protein-synthetic nanodisc
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Characteristics
- Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
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Purinergic Receptor P2Y, G-Protein Coupled, 10 (P2RY10) (AA 1-328) protein (His tag)
custom-made P2RY10 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Purinergic Receptor P2Y, G-Protein Coupled, 10 (P2RY10) (AA 1-339) protein (His tag)custom-made P2RY10 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Purinergic Receptor P2Y, G-Protein Coupled, 10 (P2RY10) (AA 1-339) protein (GST tag)P2RY10 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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Comment
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Advantages of Synthetic Nanodiscs:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Intolerant to acids and high concentrations of divalent metal ions
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
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Expiry Date
- 12 months
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- P2RY10 (Purinergic Receptor P2Y, G-Protein Coupled, 10 (P2RY10))
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Alternative Name
- P2Y10
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Background
- The protein encoded by this gene belongs to the family of G-protein coupled receptors that are preferentially activated by adenosine and uridine nucleotides. There is a pseudogene for this gene nearby on chromosome X. Multiple alternatively spliced transcripts have been observed. [provided by RefSeq, Apr 2016]
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Molecular Weight
- The human full length P2Y10 protein has a MW of 38.8kDa
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UniProt
- O00398
Target
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