QRFPR Protein
Quick Overview for QRFPR Protein (ABIN7538504)
Target
Protein Type
Origin
Source
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Purpose
- Human QRFPR full length protein-synthetic nanodisc
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Characteristics
- Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
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Pyroglutamylated RFamide Peptide Receptor (QRFPR) (AA 1-431) protein (His tag)
custom-made QRFPR Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Pyroglutamylated RFamide Peptide Receptor (QRFPR) (AA 1-433) protein (His tag)custom-made QRFPR Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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Comment
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Advantages of Synthetic Nanodiscs:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Intolerant to acids and high concentrations of divalent metal ions
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
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Expiry Date
- 12 months
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- QRFPR (Pyroglutamylated RFamide Peptide Receptor (QRFPR))
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Alternative Name
- QRFPR
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Background
- Receptor for the orexigenic neuropeptide QRFP. The activity of this receptor is mediated by G proteins that modulate adenylate cyclase activity and intracellular calcium levels.[UniProtKB/Swiss-Prot Function]
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Molecular Weight
- The human full length QRFPR protein has a MW of 49.5kDa
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UniProt
- Q96P65
Target
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