Pyrophosphatase (Inorganic) 1 (PPA1) protein (His tag)

Catalog No. ABIN7540355

This Recombinant Pyrophosphatase (Inorganic) 1 protein is expressed in Yeast (Hansenula).

Quick Overview for Pyrophosphatase (Inorganic) 1 (PPA1) protein (His tag) (ABIN7540355)

Target: Pyrophosphatase (Inorganic) 1 (PPA1)

Protein Type: Recombinant

Origin: Saccharomyces cerevisiae

Source: Yeast (Hansenula)

Application: SDS-PAGE (SDS), Enzyme Linked Inorganic Phosphate Assay (ELIPA), In vitro Transcription (IVT)

Purity: > 95 % (SDS-PAGE)

Grade: Animal-Free

Product Details

Purification tag / Conjugate: His tag

Purpose: Recombinant S. cerevisiae hydrolase, his-tagged

Sequence: MHHHHHHTYT TRQIGAKNTL EYKVYIEKDG KPVSAFHDIP LYADKENNIF NMVVEIPRWT NAKLEITKEE TLNPIIQDTK KGKLRFVRNC FPHHGYIHNY GAFPQTWEDP NVSHPETKAV GDNDPIDVLE IGETIAYTGQ VKQVKALGIM ALLDEGETDW KVIAIDINDP LAPKLNDIED VEKYFPGLLR ATNEWFRIYK IPDGKPENQF AFSGEAKNKK YALDIIKETH DSWKQLIAGK SSDSKGIDLT NVTLPDTPTY SKAASDAIPP ASPKADAPID KSIDKWFFIS GSV

Specificity: Inorganic Pyrophosphatase (iPPase, EC 3.6.1.1) is a recombinant hydrolase cloned from S. cerevisiae and overexpressed in H. polymorpha. The enzyme is active as dimer and requires divalent cations such as Mg 2+ to catalyse the reaction. iPPase can pull energy consuming reactions requiring ATP hydrolysis by hydrolizing the resulting diphosphate e.g. during in vitro RNA synthesis.

Purification: multi-step purification including chromatography and diafiltration

Unit Definition: One unit is defined as the amount of Enzyme necessary to hydrolyse 1 μmol diphosphate in one minute at pH 7.2 and 25 °C which is measured as phosphate released according to the Malachite Green reaction.

Application Details

Application Notes: use about 20,000-fold diluted (10 μU/μl) in assay: dilute 2,000-fold and add 0.1 volume diluted enzyme to 0.9 volume diphosphate assay reaction

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 25 mM NaCl, 0.1 mM EDTA, 50 % v/v glycerol, 10 mM TRIS-HCl, pH 7.5 (at 25 °C)

Storage: -20 °C

Target Details

Target: Pyrophosphatase (Inorganic) 1 (PPA1)

Alternative Name: inorganic Pyrophosphatase

Background: Inorganic Pyrophosphatase (iPPase, EC 3.6.1.1) is a recombinant hydrolase cloned from S. cerevisiae and overexpressed in H. polymorpha. The enzyme is active as dimer and requires divalent cations such as Mg 2+ to catalyse the reaction. iPPase can pull energy consuming reactions requiring ATP hydrolysis by hydrolizing the resulting diphosphate e.g. during in vitro RNA synthesis.

Molecular Weight: theor. 33.1 kDa (monomer)

Gene ID: 852296

NCBI Accession: NP_009565

UniProt: P00817