TRIM45 Protein (AA 1-580) (His tag)

Catalog No. ABIN7544680

This Recombinant TRIM45 protein is produced in HEK-293 Cells.

Quick Overview for TRIM45 Protein (AA 1-580) (His tag) (ABIN7544680)

Target: TRIM45 (Tripartite Motif Containing 45 (TRIM45))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-580

Purification tag / Conjugate: This TRIM45 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant TRIM45 Protein expressed in mammalian cells.

Sequence: MSENRKPLLG FVSKLTSGTA LGNSGKTHCP LCLGLFKAPR LLPCLHTVCT TCLEQLEPFS VVDIRGGDSD TSSEGSIFQE LKPRSLQSQI GILCPVCDAQ VDLPMGGVKA LTIDHLAVND VMLESLRGEG QGLVCDLCND REVEKRCQTC KANLCHFCCQ AHRRQKKTTY HTMVDLKDLK GYSRIGKPIL CPVHPAEELR LFCEFCDRPV CQDCVVGEHR EHPCDFTSNV IHKHGDSVWE LLKGTQPHVE ALEEALAQIH IINSALQKRV EAVAADVRTF SEGYIKAIEE HRDKLLKQLE DIRAQKENSL QLQKAQLEQL LADMRTGVEF TEHLLTSGSD LEILITKRVV VERLRKLNKV QYSTRPGVND KIRFCPQEKA GQCRGYEIYG TINTKEVDPA KCVLQGEDLH RAREKQTASF TLLCKDAAGE IMGRGGDNVQ VAVVPKDKKD SPVRTMVQDN KDGTYYISYT PKEPGVYTVW VCIKEQHVQG SPFTVMVRRK HRPHSGVFHC CTFCSSGGQK TARCACGGTM PGGYLGCGHG HKGHPGHPHW SCCGKFNEKS ECTWTGGQSA PRSLLRTVAL Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: TRIM45 (Tripartite Motif Containing 45 (TRIM45))

Alternative Name: TRIM45

Background: E3 ubiquitin-protein ligase TRIM45 (EC 2.3.2.27) (RING finger protein 99),FUNCTION: E3 ubiquitin-protein ligase that plays a role in the regulation of inflammatory response (PubMed:36681779). Mechanistically, mediates the 'Lys-48'-linked polyubiquitination of TAB2, a regulatory protein of the kinase TAK1, leading to its degradation via the proteasomal pathway and inhibition of the TLR-mediated inflammatory immune response (PubMed:36681779). May act as a transcriptional repressor in mitogen-activated protein kinase signaling pathway. {ECO:0000269|PubMed:15351693, ECO:0000269|PubMed:36681779}.

Molecular Weight: 64.4 kDa

UniProt: Q9H8W5

AlphaFold: Q9H8W5