TMEM97 Protein (AA 1-176) (His tag)
Quick Overview for TMEM97 Protein (AA 1-176) (His tag) (ABIN7544697)
Target
See all TMEM97 ProteinsProtein Type
Origin
Source
Purity
Grade
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Protein Characteristics
- AA 1-176
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Purification tag / Conjugate
- This TMEM97 protein is labelled with His tag.
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Purpose
- Predefined custom protein recombinant TMEM97 Protein expressed in mammalian cells.
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Sequence
- MGAPATRRCV EWLLGLYFLS HIPITLFMDL QAVLPRELYP VEFRNLLKWY AKEFKDPLLQ EPPAWFKSFL FCELVFQLPF FPIATYAFLK GSCKWIRTPA IIYSVHTMTT LIPILSTFLF EDFSKASGFK GQRPETLHER LTLVSVYAPY LLIPFILLIF MLRSPYYKYE EKRKKK Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
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Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
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Characteristics
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
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Want other Options for this Protein ?
!Discover Our Catalog and Custom Protein Service Options!ProductExpression SystemConjugateOriginPriceExpression System Wheat germConjugate GST tagOrigin HumanPrice $625.71Expression System Escherichia coli (E. coli)Conjugate His-SUMO TagOrigin HumanPrice $553.28Expression System HEK-293 CellsConjugate Myc-DYKDDDDK TagOrigin HumanPrice $956.40Your project requires further customization? Contact us and discover our custom protein solutions
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Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- The buffer composition is at the discretion of the manufacturer.
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Handling Advice
- Avoid repeated freeze-thaw cycles.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C.
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Expiry Date
- 12 months
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- TMEM97 (Transmembrane Protein 97 (TMEM97))
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Alternative Name
- TMEM97
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Background
- Sigma intracellular receptor 2 (S2R) (Sigma-2 receptor) (Sigma2 receptor) (Meningioma-associated protein 30) (MAC30) (Transmembrane protein 97),FUNCTION: Sigma-2 receptor which contributes to ameliorate dysfunctional cellular processes and slow degenerative progression by regulating cell functions including cholesterol biosynthesis/trafficking, membrane trafficking, autophagy, lipid membrane-bound protein trafficking, and receptor stabilization at the cell surface (PubMed:28559337, PubMed:19583955, PubMed:23922215, PubMed:25620095, PubMed:27378690, PubMed:34799735, PubMed:30443021, PubMed:34233061, PubMed:35970844) (Probable). Forms a ternary complex with PGRMC1 receptor and low density lipoprotein receptor/LDLR at the plasma membrane, which increases LDLR-mediated LDL cholesterol internalization (PubMed:30443021). Decreases lysosomal sterol transporter NPC1 availability to the cell, probably through NPC1-binding, hence controlling lipid transport, including cholesterol and LBPA, outside of late endosome/lysosome (PubMed:19583955, PubMed:27378690). Binds regio- and stereoselective ligand 20(S)-hydroxycholesterol (20(S)-OHC) which enhances TMEM97-NPC1 interaction and decreases TMEM97-PGRMC1 and TMEM97-TSPO interactions, thereby linking OHC binding to cholesterol homeostasis (PubMed:34799735, PubMed:37047353). Also able to bind cholesterol (By similarity). Binds histatin 1 (Hst 1)/HN1 salivary peptide at the ER membrane, which is critical for increasing mitochondria-ER contacts and stimulating Hst1 wound healing properties (PubMed:34233061, PubMed:35970844). May alter the activity of some cytochrome P450 proteins (PubMed:22292588). Although shows homologies with sterol isomerases (EXPERA domain), not able to catalyze sterol isomerization (Probable) (PubMed:34880501). However, may act as sensors of these molecules (Probable) (PubMed:34880501). Acts as a quality control factor in the ER, promoting the proteolytic degradation of nonproductive and extramitochondrial precursor proteins in the ER membrane thus removing them from the ER surface (By similarity). {ECO:0000250|UniProtKB:Q12155, ECO:0000250|UniProtKB:Q3MHW7, ECO:0000269|PubMed:19583955, ECO:0000269|PubMed:27378690, ECO:0000269|PubMed:28559337, ECO:0000269|PubMed:30443021, ECO:0000269|PubMed:34233061, ECO:0000269|PubMed:34799735, ECO:0000269|PubMed:34880501, ECO:0000269|PubMed:35970844, ECO:0000269|PubMed:37047353, ECO:0000303|PubMed:22292588, ECO:0000303|PubMed:23922215, ECO:0000303|PubMed:25620095, ECO:0000305|PubMed:25566323}.
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Molecular Weight
- 20.8 kDa
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UniProt
- Q5BJF2
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AlphaFold
- Q5BJF2
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Pathways
- SARS-CoV-2 Protein Interactome
Target
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