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TMEM97 Protein (AA 1-176) (His tag)

Recombinant TMEM97 protein expressed in HEK-293 Cells.
Catalog No. ABIN7544697

Quick Overview for TMEM97 Protein (AA 1-176) (His tag) (ABIN7544697)

Target

See all TMEM97 Proteins
TMEM97 (Transmembrane Protein 97 (TMEM97))

Protein Type

Recombinant

Origin

  • 4
  • 2
Human

Source

  • 2
  • 2
  • 1
  • 1
HEK-293 Cells

Purity

> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade

custom-made
  • Protein Characteristics

    AA 1-176

    Purification tag / Conjugate

    This TMEM97 protein is labelled with His tag.

    Purpose

    Predefined custom protein recombinant TMEM97 Protein expressed in mammalian cells.

    Sequence

    MGAPATRRCV EWLLGLYFLS HIPITLFMDL QAVLPRELYP VEFRNLLKWY AKEFKDPLLQ EPPAWFKSFL FCELVFQLPF FPIATYAFLK GSCKWIRTPA IIYSVHTMTT LIPILSTFLF EDFSKASGFK GQRPETLHER LTLVSVYAPY LLIPFILLIF MLRSPYYKYE EKRKKK Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

    Specificity

    If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

    Characteristics

    Key Benefits:
    • Predefined custom protein - from design to production - by highly experienced protein experts.
    • Protein expressed in mammalian cells and purified in one-step affinity chromatography
    • The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).
    This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    If you are not interested in a full length protein, please contact us for individual protein fragments.

    The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
  • Want other Options for this Protein ?

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    Price $553.28
    Expression System HEK-293 Cells
    Conjugate Myc-DYKDDDDK Tag
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    Price $956.40

    Your project requires further customization? Contact us and discover our custom protein solutions

  • Application Notes

    We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    The buffer composition is at the discretion of the manufacturer.

    Handling Advice

    Avoid repeated freeze-thaw cycles.

    Storage

    -80 °C

    Storage Comment

    Store at -80°C.

    Expiry Date

    12 months
  • Target

    TMEM97 (Transmembrane Protein 97 (TMEM97))

    Alternative Name

    TMEM97

    Background

    Sigma intracellular receptor 2 (S2R) (Sigma-2 receptor) (Sigma2 receptor) (Meningioma-associated protein 30) (MAC30) (Transmembrane protein 97),FUNCTION: Sigma-2 receptor which contributes to ameliorate dysfunctional cellular processes and slow degenerative progression by regulating cell functions including cholesterol biosynthesis/trafficking, membrane trafficking, autophagy, lipid membrane-bound protein trafficking, and receptor stabilization at the cell surface (PubMed:28559337, PubMed:19583955, PubMed:23922215, PubMed:25620095, PubMed:27378690, PubMed:34799735, PubMed:30443021, PubMed:34233061, PubMed:35970844) (Probable). Forms a ternary complex with PGRMC1 receptor and low density lipoprotein receptor/LDLR at the plasma membrane, which increases LDLR-mediated LDL cholesterol internalization (PubMed:30443021). Decreases lysosomal sterol transporter NPC1 availability to the cell, probably through NPC1-binding, hence controlling lipid transport, including cholesterol and LBPA, outside of late endosome/lysosome (PubMed:19583955, PubMed:27378690). Binds regio- and stereoselective ligand 20(S)-hydroxycholesterol (20(S)-OHC) which enhances TMEM97-NPC1 interaction and decreases TMEM97-PGRMC1 and TMEM97-TSPO interactions, thereby linking OHC binding to cholesterol homeostasis (PubMed:34799735, PubMed:37047353). Also able to bind cholesterol (By similarity). Binds histatin 1 (Hst 1)/HN1 salivary peptide at the ER membrane, which is critical for increasing mitochondria-ER contacts and stimulating Hst1 wound healing properties (PubMed:34233061, PubMed:35970844). May alter the activity of some cytochrome P450 proteins (PubMed:22292588). Although shows homologies with sterol isomerases (EXPERA domain), not able to catalyze sterol isomerization (Probable) (PubMed:34880501). However, may act as sensors of these molecules (Probable) (PubMed:34880501). Acts as a quality control factor in the ER, promoting the proteolytic degradation of nonproductive and extramitochondrial precursor proteins in the ER membrane thus removing them from the ER surface (By similarity). {ECO:0000250|UniProtKB:Q12155, ECO:0000250|UniProtKB:Q3MHW7, ECO:0000269|PubMed:19583955, ECO:0000269|PubMed:27378690, ECO:0000269|PubMed:28559337, ECO:0000269|PubMed:30443021, ECO:0000269|PubMed:34233061, ECO:0000269|PubMed:34799735, ECO:0000269|PubMed:34880501, ECO:0000269|PubMed:35970844, ECO:0000269|PubMed:37047353, ECO:0000303|PubMed:22292588, ECO:0000303|PubMed:23922215, ECO:0000303|PubMed:25620095, ECO:0000305|PubMed:25566323}.

    Molecular Weight

    20.8 kDa

    UniProt

    Q5BJF2

    AlphaFold

    Q5BJF2

    Pathways

    SARS-CoV-2 Protein Interactome
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