YJEFN3 Protein (AA 1-299) (His tag)

Catalog No. ABIN7545215

This Recombinant YJEFN3 protein is expressed in HEK-293 Cells.

Quick Overview for YJEFN3 Protein (AA 1-299) (His tag) (ABIN7545215)

Target: YJEFN3 (YjeF N-Terminal Domain Containing 3 (YJEFN3))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-299

Purification tag / Conjugate: This YJEFN3 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant YJEFN3 Protein expressed in mammalian cells.

Sequence: MSSAAGPDPS EAPEERHFLR ALELQPPLAD MGRAELSSNA TTSLVQRRKQ AWGRQSWLEQ IWNAGPVCQS TAEAAALERE LLEDYRFGRQ QLVELCGHAS AVAVTKAFPL PALSRKQRTV LVVCGPEQNG AVGLVCARHL RVFEYEPTIF YPTRSLDLLH RDLTTQCEKM DIPFLSYLPT EVQLINEAYG LVVDAVLGPG VEPGEVGGPC TRALATLKLL SIPLVSLDIP SGWDAETGSD SEDGLRPDVL VSLAAPKRCA GRFSGRHHFV AGRFVPDDVR RKFALRLPGY TGTDCVAAL Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: YJEFN3 (YjeF N-Terminal Domain Containing 3 (YJEFN3))

Alternative Name: YJEFN3

Background: YjeF N-terminal domain-containing protein 3 (YjeF_N3) (hYjeF_N3) (ApoA-I-binding protein 2),FUNCTION: May accelerate cholesterol efflux from endothelial cells to high-density lipoprotein (HDL) and thereby regulates angiogenesis. May orchestrate hematopoietic stem and progenitor cell emergence from the hemogenic endothelium, a type of specialized endothelium manifesting hematopoietic potential. YJEFN3-mediated cholesterol efflux activates endothelial SREBF2, the master transcription factor for cholesterol biosynthesis, which in turn transactivates NOTCH and promotes hematopoietic stem and progenitor cell emergence (By similarity). May play a role in spermiogenesis and oogenesis (PubMed:17533573). {ECO:0000250|UniProtKB:Q1LVI2, ECO:0000269|PubMed:17533573}.

Molecular Weight: 32.6 kDa

UniProt: A6XGL0

AlphaFold: A6XGL0