ZWINT Protein (AA 1-277) (His tag)
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- Target See all ZWINT Proteins
- ZWINT (ZW10 Interactor (ZWINT))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-277
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ZWINT protein is labelled with His tag.
- Purpose
- Made-to-order recombinant ZWINT Protein expressed in mammalian cells.
- Sequence
- MEAAETEAEA AALEVLAEVA GILEPVGLQE EAELPAKILV EFVVDSQKKD KLLCSQLQVA DFLQNILAQE DTAKGLDPLA SEDTSRQKAI AAKEQWKELK ATYREHVEAI KIGLTKALTQ MEEAQRKRTQ LREAFEQLQA KKQMAMEKRR AVQNQWQLQQ EKHLQHLAEV SAEVRERKTG TQQELDRVFQ KLGNLKQQAE QERDKLQRYQ TFLQLLYTLQ GKLLFPEAEA EAENLPDDKP QQPTRPQEQS TGDTMGRDPG VSFKAVGLQP AGDVNLP Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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ZW10 Interactor (ZWINT) (AA 1-277) protein (GST tag)
ZWINT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
ZW10 Interactor (ZWINT) (AA 1-230) protein (His tag)ZWINT Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
ZW10 Interactor (ZWINT) (Transcript Variant 3) protein (His tag)ZWINT Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
ZW10 Interactor (ZWINT) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)ZWINT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
ZW10 Interactor (ZWINT) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)ZWINT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- ZWINT (ZW10 Interactor (ZWINT))
- Alternative Name
- ZWINT (ZWINT Products)
- Background
- ZW10 interactor (ZW10-interacting protein 1) (Zwint-1),FUNCTION: Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase. {ECO:0000269|PubMed:15094189, ECO:0000269|PubMed:15485811, ECO:0000269|PubMed:15824131, ECO:0000269|PubMed:16732327}.
- Molecular Weight
- 31.3 kDa
- UniProt
- O95229
- AlphaFold
- O95229
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