ZWINT Protein (His tag)
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- Target See all ZWINT Proteins
- ZWINT (ZW10 Interactor (ZWINT))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This ZWINT protein is labelled with His tag.
- Purpose
- Recombinant Human ZWINT Protein (His Tag)
- Sequence
- Met 1-Pro277
- Characteristics
- Recombinant Human ZW10 Interactor is produced by our E.coli expression system and the target gene encoding Met1-Pro277 is expressed with a 6His tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product ZWINT Protein
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ZW10 Interactor (ZWINT) (AA 1-277) protein (GST tag)
ZWINT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
ZW10 Interactor (ZWINT) (AA 1-230) protein (His tag)ZWINT Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
ZW10 Interactor (ZWINT) (Transcript Variant 3) protein (His tag)ZWINT Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
ZW10 Interactor (ZWINT) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)ZWINT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
ZW10 Interactor (ZWINT) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)ZWINT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- ZWINT (ZW10 Interactor (ZWINT))
- Alternative Name
- ZWINT (ZWINT Products)
- Synonyms
- ZWINT Protein, HZwint-1 Protein, KNTC2AP Protein, ZWINT1 Protein, 2010007E07Rik Protein, 2600001N01Rik Protein, D10Ertd749e Protein, Zwint-1 Protein, Sip30 Protein, ZW10 interacting kinetochore protein Protein, ZW10 interactor Protein, ZWINT Protein, Zwint Protein
- Background
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Background: ZW10 Interactor is localized to the kinetochores from late Prophase to Anaphase and has a uniform distribution in the cytoplasm of Interphase cells. ZWINT interacts ZW10, MIS12 and NDC80 subunit of the NDC80 complex specifically during mitosis. ZWINT is a part of the MIS12 complex which is required for kinetochore formation and spindle checkpoint activity. In addition, ZWINT is required to target ZW10 to the kinetochore at prometaphase.
Synonym: ZW10 Interactor, ZW10-Interacting Protein 1, Zwint-1, ZWINT
- Molecular Weight
- 33.4 kDa
- UniProt
- O95229
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