SLFN12 Protein (AA 1-578) (His tag)

Catalog No. ABIN7546206

Recombinant SLFN12 protein expressed in HEK-293 Cells.

Quick Overview for SLFN12 Protein (AA 1-578) (His tag) (ABIN7546206)

Target: SLFN12 (Schlafen Family Member 12 (SLFN12))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-578

Purification tag / Conjugate: This SLFN12 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant SLFN12 Protein expressed in mammalian cells.

Sequence: MNISVDLETN YAELVLDVGR VTLGENSRKK MKDCKLRKKQ NESVSRAMCA LLNSGGGVIK AEIENEDYSY TKDGIGLDLE NSFSNILLFV PEYLDFMQNG NYFLIFVKSW SLNTSGLRIT TLSSNLYKRD ITSAKVMNAT AALEFLKDMK KTRGRLYLRP ELLAKRPCVD IQEENNMKAL AGVFFDRTEL DRKEKLTFTE STHVEIKNFS TEKLLQRIKE ILPQYVSAFA NTDGGYLFIG LNEDKEIIGF KAEMSDLDDL EREIEKSIRK MPVHHFCMEK KKINYSCKFL GVYDKGSLCG YVCALRVERF CCAVFAKEPD SWHVKDNRVM QLTRKEWIQF MVEAEPKFSS SYEEVISQIN TSLPAPHSWP LLEWQRQRHH CPGLSGRITY TPENLCRKLF LQHEGLKQLI CEEMDSVRKG SLIFSRSWSV DLGLQENHKV LCDALLISQD SPPVLYTFHM VQDEEFKGYS TQTALTLKQK LAKIGGYTKK VCVMTKIFYL SPEGMTSCQY DLRSQVIYPE SYYFTRRKYL LKALFKALKR LKSLRDQFSF AENLYQIIGI DCFQKNDKKM FKSCRRLT Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SLFN12 (Schlafen Family Member 12 (SLFN12))

Alternative Name: SLFN12

Background: Ribonuclease SLFN12 (EC 3.1.-.-) (Schlafen family member 12),FUNCTION: Ribonuclease which is part of an E2/17beta-estradiol-induced pro-apoptotic signaling pathway. E2 stabilizes the PDE3A/SLFN12 complex in the cytosol, promoting the dephosphorylation of SLFN12 and activating its pro-apoptotic ribosomal RNA/rRNA ribonuclease activity. This apoptotic pathway might be relevant in tissues with high concentration of E2 and be for instance involved in placenta remodeling (PubMed:31420216, PubMed:35104454, PubMed:34272366, PubMed:34707099). May play a role in cell differentiation (PubMed:30045019). {ECO:0000269|PubMed:30045019, ECO:0000269|PubMed:31420216, ECO:0000269|PubMed:34272366, ECO:0000269|PubMed:34707099, ECO:0000269|PubMed:35104454}.

Molecular Weight: 67.0 kDa

UniProt: Q8IYM2

AlphaFold: Q8IYM2