SP8 Protein (AA 1-490) (His tag)
custom-made
SP8
Origin: Human
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all SP8 Proteins
- SP8 (Sp8 Transcription Factor (SP8))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-490
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SP8 protein is labelled with His tag.
- Purpose
- Predefined custom protein recombinant SP8 Protein expressed in mammalian cells.
- Sequence
- MLAATCNKIG SPSPSPSSLS DSSSSFGKGF HPWKRSSSSS SASCNVVGSS LSSFGVSGAS RNGGSSSAAA AAAAAAAAAA ALVSDSFSCG GSPGSSAFSL TSSSAAAAAA AAAAAASSSP FANDYSVFQA PGVSGGSGGG GGGGGGGSSA HSQDGSHQPV FISKVHTSVD GLQGIYPRVG MAHPYESWFK PSHPGLGAAG EVGSAGASSW WDVGAGWIDV QNPNSAAALP GSLHPAAGGL QTSLHSPLGG YNSDYSGLSH SAFSSGASSH LLSPAGQHLM DGFKPVLPGS YPDSAPSPLA GAGGSMLSAG PSAPLGGSPR SSARRYSGRA TCDCPNCQEA ERLGPAGASL RRKGLHSCHI PGCGKVYGKT SHLKAHLRWH TGERPFVCNW LFCGKRFTRS DELQRHLRTH TGEKRFACPV CNKRFMRSDH LSKHVKTHSG GGGGGGSAGS GSGGKKGSDT DSEHSAAGSP PCHSPELLQP PEPGHRNGLE Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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Sp8 Transcription Factor (SP8) (AA 1-486) protein (His tag)
custom-made SP8 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- SP8 (Sp8 Transcription Factor (SP8))
- Alternative Name
- SP8 (SP8 Products)
- Background
- Transcription factor Sp8 (Specificity protein 8),FUNCTION: Transcription factor which plays a key role in limb development. Positively regulates FGF8 expression in the apical ectodermal ridge (AER) and contributes to limb outgrowth in embryos (By similarity). {ECO:0000250}.
- Molecular Weight
- 48.7 kDa
- UniProt
- Q8IXZ3
- AlphaFold
- Q8IXZ3
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