SRPRA (AA 1-638) protein (His tag)

Catalog No. ABIN7546281

Recombinant SRPRA protein expressed in HEK-293 Cells.

Quick Overview for SRPRA (AA 1-638) protein (His tag) (ABIN7546281)

Target: SRPRA

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-638

Purification tag / Conjugate: His tag

Purpose: Predefined custom protein recombinant SRPRA Protein expressed in mammalian cells.

Sequence: MLDFFTIFSK GGLVLWCFQG VSDSCTGPVN ALIRSVLLQE RGGNNSFTHE ALTLKYKLDN QFELVFVVGF QKILTLTYVD KLIDDVHRLF RDKYRTEIQQ QSALSLLNGT FDFQNDFLRL LREAEESSKI RAPTTMKKFE DSEKAKKPVR SMIETRGEKP KEKAKNSKKK GAKKEGSDGP LATSKPVPAE KSGLPVGPEN GVELSKEELI RRKREEFIQK HGRGMEKSNK STKSDAPKEK GKKAPRVWEL GGCANKEVLD YSTPTTNGTP EAALSEDINL IRGTGSGGQL QDLDCSSSDD EGAAQNSTKP SATKGTLGGM FGMLKGLVGS KSLSREDMES VLDKMRDHLI AKNVAADIAV QLCESVANKL EGKVMGTFST VTSTVKQALQ ESLVQILQPQ RRVDMLRDIM DAQRRQRPYV VTFCGVNGVG KSTNLAKISF WLLENGFSVL IAACDTFRAG AVEQLRTHTR RLSALHPPEK HGGRTMVQLF EKGYGKDAAG IAMEAIAFAR NQGFDVVLVD TAGRMQDNAP LMTALAKLIT VNTPDLVLFV GEALVGNEAV DQLVKFNRAL ADHSMAQTPR LIDGIVLTKF DTIDDKVGAA ISMTYITSKP IVFVGTGQTY CDLRSLNAKA VVAALMKA Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SRPRA

Background: Signal recognition particle receptor subunit alpha (SR-alpha) (Docking protein alpha) (DP-alpha),FUNCTION: Component of the signal recognition particle (SRP) complex receptor (SR) (PubMed:16439358). Ensures, in conjunction with the SRP complex, the correct targeting of the nascent secretory proteins to the endoplasmic reticulum membrane system (PubMed:16675701, PubMed:34020957). Forms a guanosine 5'-triphosphate (GTP)-dependent complex with the SRP subunit SRP54 (PubMed:34020957). SRP receptor compaction and GTPase rearrangement drive SRP-mediated cotranslational protein translocation into the ER (PubMed:34020957). {ECO:0000269|PubMed:16439358, ECO:0000269|PubMed:16675701, ECO:0000269|PubMed:34020957}.

Molecular Weight: 69.8 kDa

UniProt: P08240

AlphaFold: P08240