CYP3A4 Protein (AA 1-503) (His tag)
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- Target See all CYP3A4 Proteins
- CYP3A4 (Cytochrome P450, Family 3, Subfamily A, Polypeptide 4 (CYP3A4))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-503
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CYP3A4 protein is labelled with His tag.
- Purpose
- Made-to-order recombinant CYP3A43 Protein expressed in mammalian cells.
- Sequence
- MDLIPNFAME TWVLVATSLV LLYIYGTHSH KLFKKLGIPG PTPLPFLGTI LFYLRGLWNF DRECNEKYGE MWGLYEGQQP MLVIMDPDMI KTVLVKECYS VFTNQMPLGP MGFLKSALSF AEDEEWKRIR TLLSPAFTSV KFKEMVPIIS QCGDMLVRSL RQEAENSKSI NLKDFFGAYT MDVITGTLFG VNLDSLNNPQ DPFLKNMKKL LKLDFLDPFL LLISLFPFLT PVFEALNIGL FPKDVTHFLK NSIERMKESR LKDKQKHRVD FFQQMIDSQN SKETKSHKAL SDLELVAQSI IIIFAAYDTT STTLPFIMYE LATHPDVQQK LQEEIDAVLP NKAPVTYDAL VQMEYLDMVV NETLRLFPVV SRVTRVCKKD IEINGVFIPK GLAVMVPIYA LHHDPKYWTE PEKFCPERFS KKNKDSIDLY RYIPFGAGPR NCIGMRFALT NIKLAVIRAL QNFSFKPCKE TQIPLKLDNL PILQPEKPIV LKVHLRDGIT SGP Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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Cytochrome P450, Family 3, Subfamily A, Polypeptide 4 (CYP3A4) (AA 244-503) protein (His tag)
CYP3A4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Cytochrome P450, Family 3, Subfamily A, Polypeptide 4 (CYP3A4) (AA 404-503) protein (GST tag)CYP3A4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Cytochrome P450, Family 3, Subfamily A, Polypeptide 4 (CYP3A4) (AA 1-503) protein (GST tag)CYP3A4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Cytochrome P450, Family 3, Subfamily A, Polypeptide 4 (CYP3A4) (AA 1-280) protein (GST tag)CYP3A4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Cytochrome P450, Family 3, Subfamily A, Polypeptide 4 (CYP3A4) protein (Myc-DYKDDDDK Tag)CYP3A4 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- CYP3A4 (Cytochrome P450, Family 3, Subfamily A, Polypeptide 4 (CYP3A4))
- Alternative Name
- CYP3A43 (CYP3A4 Products)
- Background
- Cytochrome P450 3A43 (EC 1.14.14.1),FUNCTION: Exhibits low testosterone 6-beta-hydroxylase activity.
- Molecular Weight
- 57.7 kDa
- UniProt
- Q9HB55
- AlphaFold
- Q9HB55
- Pathways
- Steroid Hormone Biosynthesis
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