CRADD Protein (AA 1-199) (His tag)
custom-made
CRADD
Origin: Human
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all CRADD Proteins
- CRADD (CASP2 and RIPK1 Domain Containing Adaptor with Death Domain (CRADD))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-199
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CRADD protein is labelled with His tag.
- Purpose
- Custom-made recombinant CRADD Protein expressed in mammalian cells.
- Sequence
- MEARDKQVLR SLRLELGAEV LVEGLVLQYL YQEGILTENH IQEINAQTTG LRKTMLLLDI LPSRGPKAFD TFLDSLQEFP WVREKLKKAR EEAMTDLPAG DRLTGIPSHI LNSSPSDRQI NQLAQRLGPE WEPMVLSLGL SQTDIYRCKA NHPHNVQSQV VEAFIRWRQR FGKQATFQSL HNGLRAVEVD PSLLLHMLE Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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CASP2 and RIPK1 Domain Containing Adaptor with Death Domain (CRADD) (AA 1-199) protein (GST tag)
CRADD Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
CASP2 and RIPK1 Domain Containing Adaptor with Death Domain (CRADD) (AA 1-199), (full length) protein (His-SUMO Tag)CRADD Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- CRADD (CASP2 and RIPK1 Domain Containing Adaptor with Death Domain (CRADD))
- Alternative Name
- CRADD (CRADD Products)
- Background
- Death domain-containing protein CRADD (Caspase and RIP adapter with death domain) (RIP-associated protein with a death domain),FUNCTION: Adapter protein that associates with PIDD1 and the caspase CASP2 to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis (PubMed:9044836, PubMed:15073321, PubMed:16652156, PubMed:17159900, PubMed:17289572). Also recruits CASP2 to the TNFR-1 signaling complex through its interaction with RIPK1 and TRADD and may play a role in the tumor necrosis factor-mediated signaling pathway (PubMed:8985253). {ECO:0000269|PubMed:15073321, ECO:0000269|PubMed:16652156, ECO:0000269|PubMed:17159900, ECO:0000269|PubMed:17289572, ECO:0000269|PubMed:8985253, ECO:0000269|PubMed:9044836}.
- Molecular Weight
- 22.7 kDa
- UniProt
- P78560
- Pathways
- Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
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