CRADD Protein (AA 1-199)

Catalog No. ABIN1692145

Product Details

Target: CRADD (CASP2 and RIPK1 Domain Containing Adaptor with Death Domain (CRADD))

Protein Type: Recombinant

Protein Characteristics: AA 1-199

Origin: Human

Source: Escherichia coli (E. coli)

Purpose: Recombinant Human Death Domain-Containing Protein CRADD/CRADD

Sequence: GSHMEARDKQ VLRSLRLELG AEVLVEGLVL QYLYQEGILT ENHIQEINAQ TTGLRKTMLL LDILPSRGPK AFDTFLDSLQ EFPWVREKLK KAREEAMTDL PAGDRLTGIP SHILNSSPSD RQINQLAQRL GPEWEPMVLS LGLSQTDIYR CKANHPHNVQ SQVVEAFIRW RQRFGKQATF QSLHNGLRAV EVDPSLLLHM LE

Characteristics: Recombinant Human Death Domain-Containing Protein CRADD/CRADD is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Glu199) of Human CRADD.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: CRADD (CASP2 and RIPK1 Domain Containing Adaptor with Death Domain (CRADD))

Alternative Name: CRADD (CRADD Products)

Synonyms: cradd Protein, MGC89035 Protein, zgc:101717 Protein, CRADD Protein, DKFZp468A2219 Protein, LOC100226369 Protein, raidd Protein, MGC78788 Protein, MRT34 Protein, RAIDD Protein, CASP2 and RIPK1 domain containing adaptor with death domain Protein, CASP2 and RIPK1 domain containing adaptor with death, gene 1 Protein, CASP2 and RIPK1 domain containing adaptor with death, gene 2 S homeolog Protein, CRADD Protein, cradd.1 Protein, cradd Protein, cradd.2.S Protein, Cradd Protein

Background: Death Domain-Containing Protein CRADD (CRADD) is widely expressed in most tissues, with particularly high expression in the adult heart, testis, liver, skeletal muscle, fetal liver, and kidney. CRADD contains one CARD domain that mediates the interaction with caspase-2, and one death domain involved in the binding of RIP protein. CRADD functions as an apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. CRADD induces cell apoptosis/cell death in numerous tissues. Defects in CRADD will result in mental retardation.
Alternative Names: Death Domain-Containing Protein CRADD, Caspase and RIP Adapter with Death Domain, RIP-Associated Protein with A Death Domain, CRADD, RAIDD

Molecular Weight: 23 kDa

UniProt: P78560

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity