CRADD Protein (His tag)

Catalog No. ABIN7317332

This Recombinant CRADD protein is expressed in Escherichia coli (E. coli).

Quick Overview for CRADD Protein (His tag) (ABIN7317332)

Target: CRADD (CASP2 and RIPK1 Domain Containing Adaptor with Death Domain (CRADD))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This CRADD protein is labelled with His tag.

Purpose: Recombinant Human CRADD/RAIDD Protein (His Tag)

Sequence: Met 1-Glu 199

Characteristics: A DNA sequence encoding the human CRADD (P78560) (Met 1-Glu 199) was fused with a polyhistidine tag at the C-terminus.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, 20 % glycerol, pH 8.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CRADD (CASP2 and RIPK1 Domain Containing Adaptor with Death Domain (CRADD))

Alternative Name: CRADD/RAIDD

Background:

Background: Death domain-containing protein CRADD; also known as Caspase and RIP adapter with death domain; RIP-associated protein with a death domain; CRADD and RAIDD; is a protein which is constitutively expressed in most tissues; with particularly high expression in adult heart; testis; liver; skeletal muscle; fetal liver and kidney. CRADD / RAIDD contains one CARD domain and one death domain. CRADD / RAIDD contains a death domain involved in the binding of RIP protein. The CARD domain mediates the interaction with caspase-2. FADD / MORT1 is a death domain (DD)-containing adaptor / signaling molecule that interacts with the intracellular DD of FAS / APO-I ( CD95 ) and tumor necrosis factor receptor 1 and the prodomain of caspase-8 ( Mch5 / MACH / FLICE). CRADD / RAIDD has a dual-domain structure similar to that of FADD. CRADD / RAIDD has an NH2-terminal caspase homology domain that interacts with caspase-2 and a COOH-terminal DD that interacts with RIP. CRADD / RAIDD could play a role in regulating apoptosis in mammalian cells. CRADD / RAIDD is a apoptotic adaptor molecule specific for caspase-2 and FASL / TNF receptor-interacting protein RIP. In the presence of RIP and TRADD; CRADD / RAIDD recruits caspase-2 to the TNFR-1 signalling complex.

Synonym: Death Domain-Containing Protein CRADD; Caspase and RIP Adapter with Death Domain; RIP-Associated Protein with A Death Domain; CRADD; RAIDD

Molecular Weight: 24.1 kDa

UniProt: P78560

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity