CRADD Protein (His tag)

Catalog No. ABIN7317332

Product Details

Target: CRADD (CASP2 and RIPK1 Domain Containing Adaptor with Death Domain (CRADD))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This CRADD protein is labelled with His tag.

Purpose: Recombinant Human CRADD/RAIDD Protein (His Tag)

Sequence: Met 1-Glu 199

Characteristics: A DNA sequence encoding the human CRADD (P78560) (Met 1-Glu 199) was fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, 20 % glycerol, pH 8.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CRADD (CASP2 and RIPK1 Domain Containing Adaptor with Death Domain (CRADD))

Alternative Name: CRADD/RAIDD (CRADD Products)

Synonyms: cradd Protein, MGC89035 Protein, zgc:101717 Protein, CRADD Protein, DKFZp468A2219 Protein, LOC100226369 Protein, raidd Protein, MGC78788 Protein, MRT34 Protein, RAIDD Protein, CASP2 and RIPK1 domain containing adaptor with death domain Protein, CASP2 and RIPK1 domain containing adaptor with death, gene 1 Protein, CASP2 and RIPK1 domain containing adaptor with death, gene 2 S homeolog Protein, CRADD Protein, cradd.1 Protein, cradd Protein, cradd.2.S Protein, Cradd Protein

Background:

Background: Death domain-containing protein CRADD; also known as Caspase and RIP adapter with death domain; RIP-associated protein with a death domain; CRADD and RAIDD; is a protein which is constitutively expressed in most tissues; with particularly high expression in adult heart; testis; liver; skeletal muscle; fetal liver and kidney. CRADD / RAIDD contains one CARD domain and one death domain. CRADD / RAIDD contains a death domain involved in the binding of RIP protein. The CARD domain mediates the interaction with caspase-2. FADD / MORT1 is a death domain (DD)-containing adaptor / signaling molecule that interacts with the intracellular DD of FAS / APO-I ( CD95 ) and tumor necrosis factor receptor 1 and the prodomain of caspase-8 ( Mch5 / MACH / FLICE). CRADD / RAIDD has a dual-domain structure similar to that of FADD. CRADD / RAIDD has an NH2-terminal caspase homology domain that interacts with caspase-2 and a COOH-terminal DD that interacts with RIP. CRADD / RAIDD could play a role in regulating apoptosis in mammalian cells. CRADD / RAIDD is a apoptotic adaptor molecule specific for caspase-2 and FASL / TNF receptor-interacting protein RIP. In the presence of RIP and TRADD; CRADD / RAIDD recruits caspase-2 to the TNFR-1 signalling complex.

Synonym: Death Domain-Containing Protein CRADD; Caspase and RIP Adapter with Death Domain; RIP-Associated Protein with A Death Domain; CRADD; RAIDD

Molecular Weight: 24.1 kDa

UniProt: P78560

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity