CRADD Protein (AA 1-199) (His tag)

Catalog No. ABIN7562671

This Recombinant CRADD protein is produced in HEK-293 Cells.

Quick Overview for CRADD Protein (AA 1-199) (His tag) (ABIN7562671)

Target: CRADD (CASP2 and RIPK1 Domain Containing Adaptor with Death Domain (CRADD))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-199

Purification tag / Conjugate: This CRADD protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Cradd Protein expressed in mammalian cells.

Sequence: MEARDKQVLR SLRLELGAEV LVEGLVLQYL YQEGILTENH IQEIKAQTTG LRKTMLLLDI LPSRGPKAFD TFLDSLQEFP WVREKLEKAR EEVTAELPTG DWMAGIPSHI LSSSPSDQQI NQLAQRLGPE WEPVVLSLGL SQTDIYRCKA NHPHNVHSQV VEAFVRWRQR FGKQATFLSL HKGLQAVEAD PSLLQHMLE Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: CRADD (CASP2 and RIPK1 Domain Containing Adaptor with Death Domain (CRADD))

Alternative Name: Cradd

Background: Death domain-containing protein CRADD (Caspase and RIP adapter with death domain) (RIP-associated protein with a death domain),FUNCTION: Adapter protein that associates with PIDD1 and the caspase CASP2 to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis. Also recruits CASP2 to the TNFR-1 signaling complex through its interaction with RIPK1 and TRADD and may play a role in the tumor necrosis factor-mediated signaling pathway. {ECO:0000250|UniProtKB:P78560}.

Molecular Weight: 22.7 kDa

UniProt: O88843

AlphaFold: O88843

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity