GNB3 Protein (AA 1-340) (His tag)
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- Target See all GNB3 Proteins
- GNB3 (Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 3 (GNB3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-340
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This GNB3 protein is labelled with His tag.
- Purpose
- Predefined custom protein recombinant GNB3 Protein expressed in mammalian cells.
- Sequence
- MGEMEQLRQE AEQLKKQIAD ARKACADVTL AELVSGLEVV GRVQMRTRRT LRGHLAKIYA MHWATDSKLL VSASQDGKLI VWDSYTTNKV HAIPLRSSWV MTCAYAPSGN FVACGGLDNM CSIYNLKSRE GNVKVSRELS AHTGYLSCCR FLDDNNIVTS SGDTTCALWD IETGQQKTVF VGHTGDCMSL AVSPDFNLFI SGACDASAKL WDVREGTCRQ TFTGHESDIN AICFFPNGEA ICTGSDDASC RLFDLRADQE LICFSHESII CGITSVAFSL SGRLLFAGYD DFNCNVWDSM KSERVGILSG HDNRVSCLGV TADGMAVATG SWDSFLKIWN Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 3 (GNB3) (AA 1-340) protein (His tag)
custom-made GNB3 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 3 (GNB3) (AA 1-340) protein (GST tag)GNB3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 3 (GNB3) (AA 1-257) protein (His tag)GNB3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, Imm, PC
Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 3 (GNB3) (AA 1-340) protein (His tag)GNB3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 80 % by SDS - PAGE SDS
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- GNB3 (Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 3 (GNB3))
- Alternative Name
- GNB3 (GNB3 Products)
- Background
- Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3 (Transducin beta chain 3),FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
- Molecular Weight
- 37.2 kDa
- UniProt
- P16520
- AlphaFold
- P16520
- Pathways
- Peptide Hormone Metabolism, Myometrial Relaxation and Contraction
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