NSDHL Protein (AA 1-373) (His tag)
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- Target See all NSDHL Proteins
- NSDHL (NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-373
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This NSDHL protein is labelled with His tag.
- Purpose
- Predefined custom protein recombinant NSDHL Protein expressed in mammalian cells.
- Sequence
- MEPAVSEPMR DQVARTHLTE DTPKVNADIE KVNQNQAKRC TVIGGSGFLG QHMVEQLLAR GYAVNVFDIQ QGFDNPQVRF FLGDLCSRQD LYPALKGVNT VFHCASPPPS SNNKELFYRV NYIGTKNVIE TCKEAGVQKL ILTSSASVIF EGVDIKNGTE DLPYAMKPID YYTETKILQE RAVLGANDPE KNFLTTAIRP HGIFGPRDPQ LVPILIEAAR NGKMKFVIGN GKNLVDFTFV ENVVHGHILA AEQLSRDSTL GGKAFHITND EPIPFWTFLS RILTGLNYEA PKYHIPYWVA YYLALLLSLL VMVISPVIQL QPTFTPMRVA LAGTFHYYSC ERAKKAMGYQ PLVTMDDAME RTVQSFRHLR RVK Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
- Top Product
- Discover our top product NSDHL Protein
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NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL) (AA 1-362) protein (His tag)
custom-made NSDHL Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL) (AA 1-373) protein (GST tag)NSDHL Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL) (AA 1-297) protein (His tag)NSDHL Origin: Human Host: Escherichia coli (E. coli) Recombinant > 80% by SDS-PAGE SDS
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- NSDHL (NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL))
- Alternative Name
- NSDHL (NSDHL Products)
- Background
- Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (EC 1.1.1.170) (Protein H105e3),FUNCTION: Catalyzes the NAD(P)(+)-dependent oxidative decarboxylation of the C4 methyl groups of 4-alpha-carboxysterols in post-squalene cholesterol biosynthesis (By similarity). Also plays a role in the regulation of the endocytic trafficking of EGFR (By similarity). {ECO:0000250|UniProtKB:Q9R1J0}.
- Molecular Weight
- 41.9 kDa
- UniProt
- Q15738
- AlphaFold
- Q15738
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