NSDHL Protein (AA 1-297) (His tag)

Catalog No. ABIN6387344

Product Details

Target: NSDHL (NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL))

Protein Type: Recombinant

Protein Characteristics: AA 1-297

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This NSDHL protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MGSMEPAVSE PMRDQVARTH LTEDTPKVNA DIEKVNQNQA KRCTVIGGSG FLGQHMVEQL LARGYAVNVF DIQQGFDNPQ VRFFLGDLCS RQDLYPALKG VNTVFHCASP PPSSNNKELF YRVNYIGTKN VIETCKEAGV QKLILTSSAS VIFEGVDIKN GTEDLPYAMK PIDYYTETKI LQERAVLGAN DPEKNFLTTA IRPHGIFGPR DPQLVPILIE AARNGKMKFV IGNGKNLVDF TFVENVVHGH ILAAEQLSRD STLGGKAFHI TNDEPIPFWT FLSRILTGLN YEAPKYHIPY

Purity: > 80 % by SDS - PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. 20  mM Tris-HCl buffer (  pH 8.0) containing 10 % glycerol 0.1M NaCl

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: NSDHL (NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL))

Alternative Name: NSDHL (NSDHL Products)

Synonyms: zgc:112474 Protein, H105E3 Protein, SDR31E1 Protein, XAP104 Protein, AI747449 Protein, Bpa Protein, Str Protein, NAD(P) dependent steroid dehydrogenase-like Protein, NAD(P) dependent steroid dehydrogenase-like L homeolog Protein, NSDHL Protein, nsdhl Protein, nsdhl.L Protein, Nsdhl Protein

Background: NSDHL, as known as Sterol-4-alpha-carboxylate 3-dehydrogenase, is involved in the production (synthesis) of cholesterol. During cholesterol synthesis, it participates in one of several steps that convert a molecule called lanosterol to cholesterol. Specifically, this enzyme removes a carbon atom and three hydrogen atoms (a methyl group) in the conversion of lanosterol to cholesterol. It is also an important component of cell membranes and myelin, the fatty covering that insulates nerve cells. Recombinant human NSDHL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Molecular Weight: 35.5kDa (320aa), confirmed by MALDI-TOF

NCBI Accession: NP_057006

UniProt: Q15738