NUP54 Protein (AA 1-507) (His tag)
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- Target See all NUP54 Proteins
- NUP54 (Nucleoporin 54kDa (NUP54))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-507
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This NUP54 protein is labelled with His tag.
- Purpose
- Made-to-order recombinant NUP54 Protein expressed in mammalian cells.
- Sequence
- MAFNFGAPSG TSGTAAATAA PAGGFGGFGT TSTTAGSAFS FSAPTNTGTT GLFGGTQNKG FGFGTGFGTT TGTSTGLGTG LGTGLGFGGF NTQQQQQTTL GGLFSQPTQA PTQSNQLINT ASALSAPTLL GDERDAILAK WNQLQAFWGT GKGYFNNNIP PVEFTQENPF CRFKAVGYSC MPSNKDEDGL VVLVFNKKET EIRSQQQQLV ESLHKVLGGN QTLTVNVEGT KTLPDDQTEV VIYVVERSPN GTSRRVPATT LYAHFEQANI KTQLQQLGVT LSMTRTELSP AQIKQLLQNP PAGVDPIIWE QAKVDNPDSE KLIPVPMVGF KELLRRLKVQ DQMTKQHQTR LDIISEDISE LQKNQTTSVA KIAQYKRKLM DLSHRTLQVL IKQEIQRKSG YAIQADEEQL RVQLDTIQGE LNAPTQFKGR LNELMSQIRM QNHFGAVRSE ERYYIDADLL REIKQHLKQQ QEGLSHLISI IKDDLEDIKL VEHGLNETIH IRGGVFS Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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Nucleoporin 54kDa (NUP54) (AA 1-510) protein (His tag)
custom-made NUP54 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Nucleoporin 54kDa (NUP54) (AA 1-507) protein (GST tag)NUP54 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Nucleoporin 54kDa (NUP54) protein (Myc-DYKDDDDK Tag)NUP54 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- NUP54 (Nucleoporin 54kDa (NUP54))
- Alternative Name
- NUP54 (NUP54 Products)
- Background
- Nucleoporin p54 (54 kDa nucleoporin),FUNCTION: Component of the nuclear pore complex, a complex required for the trafficking across the nuclear membrane. {ECO:0000250|UniProtKB:P70582}.
- Molecular Weight
- 55.4 kDa
- UniProt
- Q7Z3B4
- AlphaFold
- Q7Z3B4
- Pathways
- SARS-CoV-2 Protein Interactome
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