RFC5 Protein (AA 1-340) (His tag)
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- Target See all RFC5 Proteins
- RFC5 (Replication Factor C (Activator 1) 5, 36.5kDa (RFC5))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-340
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This RFC5 protein is labelled with His tag.
- Purpose
- Made-to-order recombinant RFC5 Protein expressed in mammalian cells.
- Sequence
- METSALKQQE QPAATKIRNL PWVEKYRPQT LNDLISHQDI LSTIQKFINE DRLPHLLLYG PPGTGKTSTI LACAKQLYKD KEFGSMVLEL NASDDRGIDI IRGPILSFAS TRTIFKKGFK LVILDEADAM TQDAQNALRR VIEKFTENTR FCLICNYLSK IIPALQSRCT RFRFGPLTPE LMVPRLEHVV EEEKVDISED GMKALVTLSS GDMRRALNIL QSTNMAFGKV TEETVYTCTG HPLKSDIANI LDWMLNQDFT TAYRNITELK TLKGLALHDI LTEIHLFVHR VDFPSSVRIH LLTKMADIEY RLSVGTNEKI QLSSLIAAFQ VTRDLIVAEA Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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Replication Factor C (Activator 1) 5, 36.5kDa (RFC5) (AA 1-339) protein (His tag)
custom-made RFC5 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Replication Factor C (Activator 1) 5, 36.5kDa (RFC5) (AA 1-340) protein (GST tag)RFC5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Replication Factor C (Activator 1) 5, 36.5kDa (RFC5) proteinRFC5 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant WB, SDS, ELISA, IP
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- RFC5 (Replication Factor C (Activator 1) 5, 36.5kDa (RFC5))
- Alternative Name
- RFC5 (RFC5 Products)
- Background
- Replication factor C subunit 5 (Activator 1 36 kDa subunit) (A1 36 kDa subunit) (Activator 1 subunit 5) (Replication factor C 36 kDa subunit) (RF-C 36 kDa subunit) (RFC36),FUNCTION: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. {ECO:0000269|PubMed:8999859}.
- Molecular Weight
- 38.5 kDa
- UniProt
- P40937
- AlphaFold
- P40937
- Pathways
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
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