CALHM1 Protein (AA 1-346) (His tag)

Catalog No. ABIN7552797

Recombinant CALHM1 protein expressed in HEK-293 Cells.

Quick Overview for CALHM1 Protein (AA 1-346) (His tag) (ABIN7552797)

Target: CALHM1 (Calcium Homeostasis Modulator 1 (CALHM1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-346

Purification tag / Conjugate: This CALHM1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant CALHM1 Protein expressed in mammalian cells.

Sequence: MMDKFRMIFQ FLQSNQESFM NGICGIMALA SAQMYSAFDF NCPCLPGYNA AYSAGILLAP PLVLFLLGLV MNNNVSMLAE EWKRPLGRRA KDPAVLRYMF CSMAQRALIA PVVWVAVTLL DGKCFLCAFC TAVPVSALGN GSLAPGLPAP ELARLLARVP CPEIYDGDWL LAREVAVRYL RCISQALGWS FVLLTTLLAF VVRSVRPCFT QAAFLKSKYW SHYIDIERKL FDETCTEHAK AFAKVCIQQF FEAMNHDLEL GHTHGTLATA PASAAAPTTP DGAEEEREKL RGITDQGTMN RLLTSWHKCK PPLRLGQEEP PLMGNGWAGG GPRPPRKEVA TYFSKV Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: CALHM1 (Calcium Homeostasis Modulator 1 (CALHM1))

Alternative Name: CALHM1

Background: Calcium homeostasis modulator protein 1 (Protein FAM26C),FUNCTION: Pore-forming subunit of a voltage-gated ion channel required for sensory perception of sweet, bitter and umami tastes (By similarity). Specifically present in type II taste bud cells, where it plays a central role in sweet, bitter and umami taste perception by inducing ATP release from the cell, ATP acting as a neurotransmitter to activate afferent neural gustatory pathways (By similarity). Together with CALHM3, forms a fast-activating voltage-gated ATP-release channel in type II taste bud cells (TBCs) (By similarity). Acts both as a voltage-gated and calcium-activated ion channel: mediates neuronal excitability in response to changes in extracellular Ca(2+) concentration (PubMed:22711817, PubMed:23300080). Has poor ion selectivity and forms a wide pore (around 14 Angstroms) that mediates permeation of Ca(2+), Na(+) and K(+), as well as permeation of monovalent anions (PubMed:22711817). Acts as an activator of the ERK1 and ERK2 cascade (PubMed:23345406). Triggers endoplasmic reticulum stress by reducing the calcium content of the endoplasmic reticulum (PubMed:21574960). May indirectly control amyloid precursor protein (APP) proteolysis and aggregated amyloid-beta (Abeta) peptides levels in a Ca(2+) dependent manner (PubMed:18585350). {ECO:0000250|UniProtKB:D3Z291, ECO:0000269|PubMed:18585350, ECO:0000269|PubMed:21574960, ECO:0000269|PubMed:22711817, ECO:0000269|PubMed:23300080, ECO:0000269|PubMed:23345406}.

Molecular Weight: 38.3 kDa

UniProt: Q8IU99

AlphaFold: Q8IU99