CAMSAP2 Protein (AA 1-1489) (His tag)

Catalog No. ABIN7552920

Recombinant CAMSAP2 protein expressed in HEK-293 Cells.

Quick Overview for CAMSAP2 Protein (AA 1-1489) (His tag) (ABIN7552920)

Target: CAMSAP2 (CAMSAP1L1) (Calmodulin Regulated Spectrin-associated Protein 1-like 1 (CAMSAP1L1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1489

Purification tag / Conjugate: This CAMSAP2 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant CAMSAP2 Protein expressed in mammalian cells.

Sequence: MGDAADPREM RKTFIVPAIK PFDHYDFSRA KIACNLAWLV AKAFGTENVP EELQEPFYTD QYDQEHIKPP VVNLLLSAEL YCRAGSLILK SDAAKPLLGH DAVIQALAQK GLYVTDQEKL VTERDLHKKP IQMSAHLAMI DTLMMAYTVE MVSIEKVIAC AQQYSAFFQA TDLPYDIEDA VMYWINKVNE HLKDIMEQEQ KLKEHHTVEA PGGQKSPSKW FWKLVPARYR KEQTLLKQLP CIPLVENLLK DGTDGCALAA LIHFYCPDVV RLEDICLKET MSLADSLYNL QLIQEFCQEY LNQCCHFTLE DMLYAASSIK SNYLVFMAEL FWWFEVVKPS FVQPRVVRPQ GAEPVKDMPS IPVLNAAKRN VLDSSSDFPS SGEGATFTQS HHHLPSRYSR PQAHSSASGG IRRSSSMSYV DGFIGTWPKE KRSSVHGVSF DISFDKEDSV QRSTPNRGIT RSISNEGLTL NNSHVSKHIR KNLSFKPING EEEAESIEEE LNIDSHSDLK SCVPLNTNEL NSNENIHYKL PNGALQNRIL LDEFGNQIET PSIEEALQII HDTEKSPHTP QPDQIANGFF LHSQEMSILN SNIKLNQSSP DNVTDTKGAL SPITDNTEVD TGIHVPSEDI PETMDEDSSL RDYTVSLDSD MDDASKFLQD YDIRTGNTRE ALSPCPSTVS TKSQPGSSAS SSSGVKMTSF AEQKFRKLNH TDGKSSGSSS QKTTPEGSEL NIPHVVAWAQ IPEETGLPQG RDTTQLLASE MVHLRMKLEE KRRAIEAQKK KMEAAFTKQR QKMGRTAFLT VVKKKGDGIS PLREEAAGAE DEKVYTDRAK EKESQKTDGQ RSKSLADIKE SMENPQAKWL KSPTTPIDPE KQWNLASPSE ETLNEGEILE YTKSIEKLNS SLHFLQQEMQ RLSLQQEMLM QMREQQSWVI SPPQPSPQKQ IRDFKPSKQA GLSSAIAPFS SDSPRPTHPS PQSSNRKSAS FSVKSQRTPR PNELKITPLN RTLTPPRSVD SLPRLRRFSP SQVPIQTRSF VCFGDDGEPQ LKESKPKEEV KKEELESKGT LEQRGHNPEE KEIKPFESTV SEVLSLPVTE TVCLTPNEDQ LNQPTEPPPK PVFPPTAPKN VNLIEVSLSD LKPPEKADVP VEKYDGESDK EQFDDDQKVC CGFFFKDDQK AENDMAMKRA ALLEKRLRRE KETQLRKQQL EAEMEHKKEE TRRKTEEERQ KKEDERARRE FIRQEYMRRK QLKLMEDMDT VIKPRPQVVK QKKQRPKSIH RDHIESPKTP IKGPPVSSLS LASLNTGDNE SVHSGKRTPR SESVEGFLSP SRCGSRNGEK DWENASTTSS VASGTEYTGP KLYKEPSAKS NKHIIQNALA HCCLAGKVNE GQKKKILEEM EKSDANNFLI LFRDSGCQFR SLYTYCPETE EINKLTGIGP KSITKKMIEG LYKYNSDRKQ FSHIPAKTLS ASVDAITIHS HLWQTKRPVT PKKLLPTKA Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: CAMSAP2 (CAMSAP1L1) (Calmodulin Regulated Spectrin-associated Protein 1-like 1 (CAMSAP1L1))

Alternative Name: CAMSAP2

Background: Calmodulin-regulated spectrin-associated protein 2 (Calmodulin-regulated spectrin-associated protein 1-like protein 1),FUNCTION: Key microtubule-organizing protein that specifically binds the minus-end of non-centrosomal microtubules and regulates their dynamics and organization (PubMed:23169647, PubMed:24486153, PubMed:24706919). Specifically recognizes growing microtubule minus-ends and autonomously decorates and stabilizes microtubule lattice formed by microtubule minus-end polymerization (PubMed:24486153, PubMed:24706919). Acts on free microtubule minus-ends that are not capped by microtubule-nucleating proteins or other factors and protects microtubule minus-ends from depolymerization (PubMed:24486153, PubMed:24706919). In addition, it also reduces the velocity of microtubule polymerization (PubMed:24486153, PubMed:24706919). Through the microtubule cytoskeleton, also regulates the organization of cellular organelles including the Golgi and the early endosomes (PubMed:27666745). Essential for the tethering, but not for nucleation of non-centrosomal microtubules at the Golgi: together with Golgi-associated proteins AKAP9 and PDE4DIP, required to tether non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed:27666745). Also acts as a regulator of neuronal polarity and development: localizes to non-centrosomal microtubule minus-ends in neurons and stabilizes non-centrosomal microtubules, which is required for neuronal polarity, axon specification and dendritic branch formation (PubMed:24908486). Through the microtubule cytoskeleton, regulates the autophagosome transport (PubMed:28726242). {ECO:0000269|PubMed:23169647, ECO:0000269|PubMed:24486153, ECO:0000269|PubMed:24706919, ECO:0000269|PubMed:24908486, ECO:0000269|PubMed:27666745, ECO:0000269|PubMed:28726242}.

Molecular Weight: 168.1 kDa

UniProt: Q08AD1

AlphaFold: Q08AD1