Ephrin A1 Protein (EFNA1) (AA 1-1120) (His tag)

Catalog No. ABIN7553761

This Recombinant Ephrin A1 protein is produced in HEK-293 Cells.

Quick Overview for Ephrin A1 Protein (EFNA1) (AA 1-1120) (His tag) (ABIN7553761)

Target: Ephrin A1 (EFNA1)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1120

Purification tag / Conjugate: This Ephrin A1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant EFL1 Protein expressed in mammalian cells.

Sequence: MVLNSLDKMI QLQKNTANIR NICVLAHVDH GKTTLADCLI SSNGIISSRL AGKLRYMDSR EDEQIRGITM KSSAISLHYA TGNEEYLINL IDSPGHVDFS SEVSTAVRIC DGCIIVVDAV EGVCPQTQAV LRQAWLENIR PVLVINKIDR LIVELKFTPQ EAYSHLKNIL EQINALTGTL FTSKVLEERA ERETESQVNP NSEQGEQVYD WSTGLEDTDD SHLYFSPEQG NVVFTSAIDG WGFGIEHFAR IYSQKIGIKK EVLMKTLWGD YYINMKAKKI MKGDQAKGKK PLFVQLILEN IWSLYDAVLK KDKDKIDKIV TSLGLKIGAR EARHSDPKVQ INAICSQWLP ISHAVLAMVC QKLPSPLDIT AERVERLMCT GSQTFDSFPP ETQALKAAFM KCGSEDTAPV IIFVSKMFAV DAKALPQNKP RPLTQEEIAQ RRERARQRHA EKLAAAQGQA PLEPTQDGSA IETCPKGEEP RGDEQQVESM TPKPVLQEEN NQESFIAFAR VFSGVARRGK KIFVLGPKYS PLEFLRRVPL GFSAPPDGLP QVPHMAYCAL ENLYLLMGRE LEYLEEVPPG NVLGIGGLQD FVLKSATLCS LPSCPPFIPL NFEATPIVRV AVEPKHPSEM PQLVKGMKLL NQADPCVQIL IQETGEHVLV TAGEVHLQRC LDDLKERFAK IHISVSEPII PFRETITKPP KVDMVNEEIG KQQKVAVIHQ MKEDQSKIPE GIQVDSDGLI TITTPNKLAT LSVRAMPLPE EVTQILEENS DLIRSMEQLT SSLNEGENTH MIHQKTQEKI WEFKGKLEQH LTGRRWRNIV DQIWSFGPRK CGPNILVNKS EDFQNSVWTG PADKASKEAS RYRDLGNSIV SGFQLATLSG PMCEEPLMGV CFVLEKWDLS KFEEQGASDL AKEGQEENET CSGGNENQEL QDGCSEAFEK RTSQKGESPL TDCYGPFSGQ LIATMKEACR YALQVKPQRL MAAMYTCDIM ATGDVLGRVY AVLSKREGRV LQEEMKEGTD MFIIKAVLPV AESFGFADEI RKRTSGLASP QLVFSHWEII PSDPFWVPTT EEEYLHFGEK ADSENQARKY MNAVRKRKGL YVEEKIVEHA EKQRTLSKNK Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Ephrin A1 (EFNA1)

Alternative Name: EFL1

Background: Elongation factor-like GTPase 1 (Elongation factor Tu GTP-binding domain-containing protein 1) (Elongation factor-like 1) (Protein FAM42A),FUNCTION: Involved in the biogenesis of the 60S ribosomal subunit and translational activation of ribosomes. Together with SBDS, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Has low intrinsic GTPase activity. GTPase activity is increased by contact with 60S ribosome subunits. {ECO:0000269|PubMed:21536732}.

Molecular Weight: 125.4 kDa

UniProt: Q7Z2Z2

AlphaFold: Q7Z2Z2

Pathways: RTK Signaling