FBXO11 Protein (AA 1-927) (His tag)

Catalog No. ABIN7553892

Recombinant FBXO11 protein expressed in HEK-293 Cells.

Quick Overview for FBXO11 Protein (AA 1-927) (His tag) (ABIN7553892)

Target: FBXO11 (F-Box Protein 11 (FBXO11))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-927

Purification tag / Conjugate: This FBXO11 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant FBXO11 Protein expressed in mammalian cells.

Sequence: MNSVRAANRR PRRVSRPRPV QQQQQQPPQQ PPPQPPQQQP PQQQPPPPPQ QQQQQQPPPP PPPPPPLPQE RNNVGERDDD VPADMVAEES GPGAQNSPYQ LRRKTLLPKR TACPTKNSME GASTSTTENF GHRAKRARVS GKSQDLSAAP AEQYLQEKLP DEVVLKIFSY LLEQDLCRAA CVCKRFSELA NDPILWKRLY MEVFEYTRPM MHPEPGKFYQ INPEEYEHPN PWKESFQQLY KGAHVKPGFA EHFYSNPARY KGRENMLYYD TIEDALGGVQ EAHFDGLIFV HSGIYTDEWI YIESPITMIG AAPGKVADKV IIENTRDSTF VFMEGSEDAY VGYMTIRFNP DDKSAQHHNA HHCLEITVNC SPIIDHCIIR STCTVGSAVC VSGQGACPTI KHCNISDCEN VGLYITDHAQ GIYEDNEISN NALAGIWVKN HGNPIIRRNH IHHGRDVGVF TFDHGMGYFE SCNIHRNRIA GFEVKAYANP TVVRCEIHHG QTGGIYVHEK GRGQFIENKI YANNFAGVWI TSNSDPTIRG NSIFNGNQGG VYIFGDGRGL IEGNDIYGNA LAGIQIRTNS CPIVRHNKIH DGQHGGIYVH EKGQGVIEEN EVYSNTLAGV WVTTGSTPVL RRNRIHSGKQ VGVYFYDNGH GVLEDNDIYN HMYSGVQIRT GSNPKIRRNK IWGGQNGGIL VYNSGLGCIE DNEIFDNAMA GVWIKTDSNP TLRRNKIHDG RDGGICIFNG GRGLLEENDI FRNAQAGVLI STNSHPILRK NRIFDGFAAG IEITNHATAT LEGNQIFNNR FGGLFLASGV NVTMKDNKIM NNQDAIEKAV SRGQCLYKIS SYTSYPMHDF YRCHTCNTTD RNAICVNCIK KCHQGHDVEF IRHDRFFCDC GAGTLSNPCT LAGEPTHDTD TLYDSAPPIE SNTLQHN Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: FBXO11 (F-Box Protein 11 (FBXO11))

Alternative Name: FBXO11

Background: F-box only protein 11 (Protein arginine N-methyltransferase 9) (Vitiligo-associated protein 1) (VIT-1),FUNCTION: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as DTL/CDT2, BCL6 and PRDM1/BLIMP1. The SCF(FBXO11) complex mediates ubiquitination and degradation of BCL6, thereby playing a role in the germinal center B-cells terminal differentiation toward memory B-cells and plasma cells. The SCF(FBXO11) complex also mediates ubiquitination and degradation of DTL, an important step for the regulation of TGF-beta signaling, cell migration and the timing of the cell-cycle progression and exit. Binds to and neddylates phosphorylated p53/TP53, inhibiting its transcriptional activity. Plays a role in the regulatiom of erythropoiesis but not myelopoiesis or megakaryopoiesis. Mechanistically, activates erythroid genes by mediating the degradation of BAHD1, a heterochromatin-associated protein that recruits corepressors to H3K27me3 marks (PubMed:33156908). Participates in macrophage cell death and inflammation in response to bacterial toxins by regulating the expression of complement 5a receptor 1/C5AR1 and IL-1beta (PubMed:33156908). Acts as a critical regulator to determine the level of MHC-II by mediating the recognition of degron at the P/S/T domain of CIITA leading to its ubiquitination and subsequent degradation via the proteasome (PubMed:37279268). Participates in the antiviral repsonse by initiating the activation of TBK1-IRF3-IFN-I axis. Mediates the 'Lys-63'-linked ubiquitination of TRAF3 to strengthen the interaction between TRAF3 and TBK1 (PubMed:36897010). {ECO:0000269|PubMed:17098746, ECO:0000269|PubMed:22113614, ECO:0000269|PubMed:23478441, ECO:0000269|PubMed:23478445, ECO:0000269|PubMed:23892434, ECO:0000269|PubMed:24613396, ECO:0000269|PubMed:33156908, ECO:0000269|PubMed:36897010, ECO:0000269|PubMed:37279268}.

Molecular Weight: 103.6 kDa

UniProt: Q86XK2

AlphaFold: Q86XK2

Pathways: Sensory Perception of Sound