Golgin A2 (GOLGA2) (AA 1-1002) protein (His tag)

Catalog No. ABIN7554026

Product Details

Target: Golgin A2 (GOLGA2)

Protein Type: Recombinant

Protein Characteristics: AA 1-1002

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: His tag

Purpose: Predefined custom protein recombinant GOLGA2 Protein expressed in mammalian cells.

Sequence: MWPQPRLPPR PAMSEETRQS KLAAAKKKLR EYQQRNSPGV PTGAKKKKKI KNGSNPETTT SGGCHSPEDT PKDNAATLQP SDDTVLPGGV PSPGASLTSM AASQNHDADN VPNLMDETKT FSSTESLRQL SQQLNGLVCE SATCVNGEGP ASSANLKDLE SRYQQLAVAL DSSYVTNKQL NITIEKLKQQ NQEITDQLEE EKKECHQKQG ALREQLQVHI QTIGILVSEK AELQTALAHT QHAARQKEGE SEDLASRLQY SRRRVGELER ALSAVSTQQK KADRYNKELT KERDALRLEL YKNTQSNEDL KQEKSELEEK LRVLVTEKAG MQLNLEELQK KLEMTELLLQ QFSSRCEAPD ANQQLQQAME ERAQLEAHLG QVMESVRQLQ MERDKYAENL KGESAMWRQR MQQMSEQVHT LREEKECSMS RVQELETSLA ELRNQMAEPP PPEPPAGPSE VEQQLQAEAE HLRKELEGLA GQLQAQVQDN EGLSRLNREQ EERLLELERA AELWGEQAEA RRQILETMQN DRTTISRALS QNRELKEQLA ELQSGFVKLT NENMEITSAL QSEQHVKREL GKKLGELQEK LSELKETVEL KSQEAQSLQQ QRDQYLGHLQ QYVAAYQQLT SEKEVLHNQL LLQTQLVDQL QQQEAQGKAV AEMARQELQE TQERLEAATQ QNQQLRAQLS LMAHPGEGDG LDREEEEDEE EEEEEAVAVP QPMPSIPEDL ESREAMVAFF NSAVASAEEE QARLRGQLKE QRVRCRRLAH LLASAQKEPE AAAPAPGTGG DSVCGETHRA LQGAMEKLQS RFMELMQEKA DLKERVEELE HRCIQLSGET DTIGEYIALY QSQRAVLKER HREKEEYISR LAQDKEEMKV KLLELQELVL RLVGDRNEWH GRFLAAAQNP ADEPTSGAPA PQELGAANQQ GDLCEVSLAG SVEPAQGEAR EGSPRDNPTA QQIMQLLREM QNPRERPGLG SNPCIPFFYR ADENDEVKIT VI Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Golgin A2 (GOLGA2)

Alternative Name: GOLGA2 (GOLGA2 Products)

Background: Golgin subfamily A member 2 (130 kDa cis-Golgi matrix protein) (GM130) (GM130 autoantigen) (Golgin-95),FUNCTION: Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane (Probable) (PubMed:16489344). Required for normal protein transport from the endoplasmic reticulum to the Golgi apparatus and the cell membrane (By similarity). Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis (By similarity). Also plays a key role in spindle pole assembly and centrosome organization (PubMed:26165940). Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle (PubMed:19242490, PubMed:26165940). Regulates the meiotic spindle pole assembly, probably via the same mechanism (By similarity). Also regulates the centrosome organization (PubMed:18045989, PubMed:19109421). Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins (PubMed:16489344, PubMed:17314401). {ECO:0000250|UniProtKB:Q62839, ECO:0000250|UniProtKB:Q921M4, ECO:0000269|PubMed:16489344, ECO:0000269|PubMed:17314401, ECO:0000269|PubMed:18045989, ECO:0000269|PubMed:19109421, ECO:0000269|PubMed:19242490, ECO:0000269|PubMed:26165940, ECO:0000305|PubMed:26363069}.

Molecular Weight: 113.1 kDa

UniProt: Q08379

AlphaFold: Q08379

Pathways: SARS-CoV-2 Protein Interactome