RALGDS Protein (AA 1-914) (His tag)

Catalog No. ABIN7554041

This Recombinant RALGDS protein is expressed in HEK-293 Cells.

Quick Overview for RALGDS Protein (AA 1-914) (His tag) (ABIN7554041)

Target: RALGDS (Ral Guanine Nucleotide Dissociation Stimulator (RALGDS))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-914

Purification tag / Conjugate: This RALGDS protein is labelled with His tag.

Purpose: Predefined custom protein recombinant RALGDS Protein expressed in mammalian cells.

Sequence: MVQRMWAEAA GPAGGAEPLF PGSRRSRSVW DAVRLEVGVP DSCPVVLHSF TQLDPDLPRP ESSTQEIGEE LINGVIYSIS LRKVQLHHGG NKGQRWLGYE NESALNLYET CKVRTVKAGT LEKLVEHLVP AFQGSDLSYV TIFLCTYRAF TTTQQVLDLL FKRYGRCDAL TASSRYGCIL PYSDEDGGPQ DQLKNAISSI LGTWLDQYSE DFCQPPDFPC LKQLVAYVQL NMPGSDLERR AHLLLAQLEH SEPIEAEPEA LSPVPALKPT PELELALTPA RAPSPVPAPA PEPEPAPTPA PGSELEVAPA PAPELQQAPE PAVGLESAPA PALELEPAPE QDPAPSQTLE LEPAPAPVPS LQPSWPSPVV AENGLSEEKP HLLVFPPDLV AEQFTLMDAE LFKKVVPYHC LGSIWSQRDK KGKEHLAPTI RATVTQFNSV ANCVITTCLG NRSTKAPDRA RVVEHWIEVA RECRILKNFS SLYAILSALQ SNSIHRLKKT WEDVSRDSFR IFQKLSEIFS DENNYSLSRE LLIKEGTSKF ATLEMNPKRA QKRPKETGII QGTVPYLGTF LTDLVMLDTA MKDYLYGRLI NFEKRRKEFE VIAQIKLLQS ACNNYSIAPD EQFGAWFRAV ERLSETESYN LSCELEPPSE SASNTLRTKK NTAIVKRWSD RQAPSTELST SGSSHSKSCD QLRCGPYLSS GDIADALSVH SAGSSSSDVE EINISFVPES PDGQEKKFWE SASQSSPETS GISSASSSTS SSSASTTPVA ATRTHKRSVS GLCNSSSALP LYNQQVGDCC IIRVSLDVDN GNMYKSILVT SQDKAPAVIR KAMDKHNLEE EEPEDYELLQ ILSDDRKLKI PENANVFYAM NSTANYDFVL KKRTFTKGVK VKHGASSTLP RMKQKGLKIA KGIF Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: RALGDS (Ral Guanine Nucleotide Dissociation Stimulator (RALGDS))

Alternative Name: RALGDS

Background: Ral guanine nucleotide dissociation stimulator (RalGDS) (Ral guanine nucleotide exchange factor) (RalGEF),FUNCTION: Functions as a guanine nucleotide exchange factor (GEF) activating either RalA or RalB GTPases and plays an important role in intracellular transport. Interacts and acts as an effector molecule for R-Ras, H-Ras, K-Ras, and Rap (By similarity). During bacterial clearance, recognizes 'Lys-33'-linked polyubiquitinated TRAF3 and subsequently mediates assembly of the exocyst complex (PubMed:27438768). {ECO:0000250|UniProtKB:Q03385, ECO:0000269|PubMed:27438768}.

Molecular Weight: 100.6 kDa

UniProt: Q12967

AlphaFold: Q12967

Pathways: Neurotrophin Signaling Pathway