NLRC4 Protein (AA 1-1024) (His tag)

Catalog No. ABIN7554754

Product Details

Target: NLRC4 (NLR Family, CARD Domain Containing 4 (NLRC4))

Protein Type: Recombinant

Protein Characteristics: AA 1-1024

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This NLRC4 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant NLRC4 Protein expressed in mammalian cells.

Sequence: MNFIKDNSRA LIQRMGMTVI KQITDDLFVW NVLNREEVNI ICCEKVEQDA ARGIIHMILK KGSESCNLFL KSLKEWNYPL FQDLNGQSLF HQTSEGDLDD LAQDLKDLYH TPSFLNFYPL GEDIDIIFNL KSTFTEPVLW RKDQHHHRVE QLTLNGLLQA LQSPCIIEGE SGKGKSTLLQ RIAMLWGSGK CKALTKFKFV FFLRLSRAQG GLFETLCDQL LDIPGTIRKQ TFMAMLLKLR QRVLFLLDGY NEFKPQNCPE IEALIKENHR FKNMVIVTTT TECLRHIRQF GALTAEVGDM TEDSAQALIR EVLIKELAEG LLLQIQKSRC LRNLMKTPLF VVITCAIQMG ESEFHSHTQT TLFHTFYDLL IQKNKHKHKG VAASDFIRSL DHCGDLALEG VFSHKFDFEL QDVSSVNEDV LLTTGLLCKY TAQRFKPKYK FFHKSFQEYT AGRRLSSLLT SHEPEEVTKG NGYLQKMVSI SDITSTYSSL LRYTCGSSVE ATRAVMKHLA AVYQHGCLLG LSIAKRPLWR QESLQSVKNT TEQEILKAIN INSFVECGIH LYQESTSKSA LSQEFEAFFQ GKSLYINSGN IPDYLFDFFE HLPNCASALD FIKLDFYGGA MASWEKAAED TGGIHMEEAP ETYIPSRAVS LFFNWKQEFR TLEVTLRDFS KLNKQDIRYL GKIFSSATSL RLQIKRCAGV AGSLSLVLST CKNIYSLMVE ASPLTIEDER HITSVTNLKT LSIHDLQNQR LPGGLTDSLG NLKNLTKLIM DNIKMNEEDA IKLAEGLKNL KKMCLFHLTH LSDIGEGMDY IVKSLSSEPC DLEEIQLVSC CLSANAVKIL AQNLHNLVKL SILDLSENYL EKDGNEALHE LIDRMNVLEQ LTALMLPWGC DVQGSLSSLL KHLEEVPQLV KLGLKNWRLT DTEIRILGAF FGKNPLKNFQ QLNLAGNRVS SDGWLAFMGV FENLKQLVFF DFSTKEFLPD PALVRKLSQV LSKLTFLQEA RLVGWQFDDD DLSVITGAFK LVTA Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: NLRC4 (NLR Family, CARD Domain Containing 4 (NLRC4))

Alternative Name: NLRC4 (NLRC4 Products)

Background: NLR family CARD domain-containing protein 4 (CARD, LRR, and NACHT-containing protein) (CED-4-like protein Clan) (Caspase recruitment domain-containing protein 12) (Ice protease-activating factor) (Ipaf),FUNCTION: Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed:15107016). The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria (By similarity). {ECO:0000250|UniProtKB:Q3UP24, ECO:0000269|PubMed:15107016}.

Molecular Weight: 116.2 kDa

UniProt: Q9NPP4

AlphaFold: Q9NPP4

Pathways: Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome