ERVK-19 Protein (AA 1-959) (His tag)

Catalog No. ABIN7555012

This Recombinant ERVK-19 protein is produced in HEK-293 Cells.

Quick Overview for ERVK-19 Protein (AA 1-959) (His tag) (ABIN7555012)

Target: ERVK-19 (Endogenous Retrovirus Group K, Member 19 (ERVK-19))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-959

Purification tag / Conjugate: This ERVK-19 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant ERVK-19 Protein expressed in mammalian cells.

Sequence: NKSKKRRNRV SFLGAATVEP PKPIPLTWKT EKPVWVNQWP LPKQKLEALH LLANEQLEKG HIEPSFSPWN SPVFVIQKKS GKWRMLTDLR AVNAVNAVIQ PMGPLQPGLP SLAMIPKDWP LIIIDLKDCF FTIPLAEQDC EKFAFTIPAI NNKEPATRFQ WKVLPQGMLN SPTICQTFVG RALQPVREKF SDCYIIHYID DILCAAEMKD KLIDCYTFLQ AEVANAGLAI ASDKIQTSTP FHYLEMQIEN RKIKPPKIEI RKDTLKTLND FQKLLGDINW IRPTLGIPTY AMSNLFSILR GDSDLNSKRM LTPEATKEIK LVEEKIQSAQ INRIDPLAPL QLLIFATAHS PTGIIIQNTD LVEWSFLPHS TVKTFTLYLD QMATLIGQTR LRIIKLCGND PDKIVVPLTK EQVRQAFINS GAWQIGLANF VGIIDNHYPK TKIFQFLKMT TWILPKITRR EPLENALTVF TDGSSNGKAA YTGPKERVIK TQYQSAQRAE LVAVITVLQD FDQPINIISD SAYVVQATRD VETALIKYSM DDQLNQLFNL LQQTVRKRNF PFYITHIRAH TNLPGPLTKA NEQADLLVSS ALIKAQELHA LTHVNVAGLK NKFDVTWKQA KDIVQHCTQC QVLHLPTQEA GVNPRGLCPN ALWQMDVTHV SSFGRLSYIH VTVDTYSHFI WATCQTGEST SHVKKHLLSC FAVMGVPEKI KTDNGPGYCS KAFQKFLSQW KISHTTGIPY NSQGQAIVER TNRTLKTQLV KQKEGGDSKE CTTPQMQLNL ALYTLNFLNI YRNQTTTSAE QHLTGKKNSP HEGKLIWWKD NKNKTWEIGK VITWGRGFAC VSPGENQLPV WIPTRHLKFY NEPIGDAKKS TSAETETPQS STVDSQDEQN GDVRRTDEVA IHQESRAADL GTTKEADAVS YKISREHKGD TNPREYAACG LDDCINGGKS PYACRSSCS Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ERVK-19 (Endogenous Retrovirus Group K, Member 19 (ERVK-19))

Alternative Name: ERVK-19

Background: Endogenous retrovirus group K member 19 Pol protein (HERV-K(C19) Pol protein) (HERV-K_19q11 provirus ancestral Pol protein) [Includes: Reverse transcriptase (RT) (EC 2.7.7.49), Ribonuclease H (RNase H) (EC 3.1.26.4), Integrase (IN)],FUNCTION: Early post-infection, the reverse transcriptase converts the viral RNA genome into double-stranded viral DNA. The RNase H domain of the reverse transcriptase performs two functions. It degrades the RNA template and specifically removes the RNA primer from the RNA/DNA hybrid. Following nuclear import, the integrase catalyzes the insertion of the linear, double-stranded viral DNA into the host cell chromosome. Endogenous Pol proteins may have kept, lost or modified their original function during evolution.

Molecular Weight: 108.1 kDa

UniProt: Q9WJR5

AlphaFold: Q9WJR5