SLFN14 Protein (AA 1-912) (His tag)

Catalog No. ABIN7555497

Recombinant SLFN14 protein expressed in HEK-293 Cells.

Quick Overview for SLFN14 Protein (AA 1-912) (His tag) (ABIN7555497)

Target: SLFN14 (Schlafen Family Member 14 (SLFN14))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-912

Purification tag / Conjugate: This SLFN14 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant SLFN14 Protein expressed in mammalian cells.

Sequence: MESLKTDTEM PYPEVIVDVG RVIFGEENRK KMTNSCLKRS ENSRIIRAIC ALLNSGGGVI KAEIDDKTYS YQCHGLGQDL ETSFQKLLPS GSQKYLDYMQ QGHNLLIFVK SWSPDVFSLP LRICSLRSNL YRRDVTSAIN LSASSALELL REKGFRAQRG RPRVKKLHPQ QVLNRCIQEE EDMRILASEF FKKDKLMYKE KLNFTESTHV EFKRFTTKKV IPRIKEMLPH YVSAFANTQG GYVLIGVDDK SKEVVGCKWE KVNPDLLKKE IENCIEKLPT FHFCCEKPKV NFTTKILNVY QKDVLDGYVC VIQVEPFCCV VFAEAPDSWI MKDNSVTRLT AEQWVVMMLD TQSAPPSLVT DYNSCLISSA SSARKSPGYP IKVHKFKEAL QRHLFPVTQE EVQFKPESLC KKLFSDHKEL EGLMKTLIHP CSQGIVIFSR SWAGDVGFRK EQNVLCDALL IAVNSPVVLY TILIDPNWPG GLEYARNTAH QLKQKLQTVG GYTGKVCIIP RLIHLSSTQS RPGEIPLRYP RSYRLADEEE MEDLLQALVV VSLSSRSLLS DQMGCEFFNL LIMEQSQLLS ESLQKTRELF IYCFPGVRKT ALAIKIMEKI KDLFHCKPKE ILYVCESDSL KDFVTQQTTC QAVTRKTFMQ GEFLKIKHIV MDETENFCSK YGNWYMKAKN ITHPKAKGTG SENLHHGILW LFLDPFQIHH ADVNGLPPPS AQFPRKTITS GIHCALEIAK VMKEEMKRIK ENPPSNMSPD TLALFSETAY EEATCAQALP GVCETKTNLT TEQIANYVAR KCHSLFQCGY LPKDIAILCR RGEDRGRYRL ALLKAMELIE THRPSEVVFS PATGVWGSHI VLDSIQQFSG LERTVVFGLS PECDQSEEFH KLCFASRAIK HLYLLYEKRA AY Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SLFN14 (Schlafen Family Member 14 (SLFN14))

Alternative Name: SLFN14

Background: Protein SLFN14 [Cleaved into: C-terminally truncated SLFN14 endoribonuclease (EC 3.1.-.-) (Schlafen family member 14)],FUNCTION: [Protein SLFN14]: Shows no ribosome-associated and endoribonuclease activities. {ECO:0000269|PubMed:25996083}., FUNCTION: [C-terminally truncated SLFN14 endoribonuclease]: Displays polysome-associated endoribonuclease activity towards mRNAs and rRNAs (PubMed:25996083). May play a role in RNA surveillance pathways by recognizing stalled ribosomes and triggering endonucleolytic cleavage of aberrant mRNAs (Probable). Cleaves different types of rRNAs and mRNAs in a magnesium- and manganese-dependent and ATP-independent manner (By similarity). Involved in correct maturation of megakaryocytes and especially important for proplatelet extension. {ECO:0000250|UniProtKB:G1SRW8, ECO:0000269|PubMed:25996083, ECO:0000269|PubMed:26769223, ECO:0000305|PubMed:25996083}.

Molecular Weight: 103.9 kDa

UniProt: P0C7P3

AlphaFold: P0C7P3