TTLL5 Protein (AA 1-1281) (His tag)

Catalog No. ABIN7555849

This Recombinant TTLL5 protein is produced in HEK-293 Cells.

Quick Overview for TTLL5 Protein (AA 1-1281) (His tag) (ABIN7555849)

Target: TTLL5 (Tubulin tyrosine Ligase-Like Family, Member 5 (TTLL5))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1281

Purification tag / Conjugate: This TTLL5 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant TTLL5 Protein expressed in mammalian cells.

Sequence: MPIVMARDLE ETASSSEDEE VISQEDHPCI MWTGGCRRIP VLVFHADAIL TKDNNIRVIG ERYHLSYKIV RTDSRLVRSI LTAHGFHEVH PSSTDYNLMW TGSHLKPFLL RTLSEAQKVN HFPRSYELTR KDRLYKNIIR MQHTHGFKAF HILPQTFLLP AEYAEFCNSY SKDRGPWIVK PVASSRGRGV YLINNPNQIS LEENILVSRY INNPLLIDDF KFDVRLYVLV TSYDPLVIYL YEEGLARFAT VRYDQGAKNI RNQFMHLTNY SVNKKSGDYV SCDDPEVEDY GNKWSMSAML RYLKQEGRDT TALMAHVEDL IIKTIISAEL AIATACKTFV PHRSSCFELY GFDVLIDSTL KPWLLEVNLS PSLACDAPLD LKIKASMISD MFTVVGFVCQ DPAQRASTRP IYPTFESSRR NPFQKPQRCR PLSASDAEMK NLVGSAREKG PGKLGGSVLG LSMEEIKVLR RVKEENDRRG GFIRIFPTSE TWEIYGSYLE HKTSMNYMLA TRLFQDRMTA DGAPELKIES LNSKAKLHAA LYERKLLSLE VRKRRRRSSR LRAMRPKYPV ITQPAEMNVK TETESEEEEE VALDNEDEEQ EASQEESAGF LRENQAKYTP SLTALVENTP KENSMKVREW NNKGGHCCKL ETQELEPKFN LMQILQDNGN LSKMQARIAF SAYLQHVQIR LMKDSGGQTF SASWAAKEDE QMELVVRFLK RASNNLQHSL RMVLPSRRLA LLERRRILAH QLGDFIIVYN KETEQMAEKK SKKKVEEEEE DGVNMENFQE FIRQASEAEL EEVLTFYTQK NKSASVFLGT HSKISKNNNN YSDSGAKGDH PETIMEEVKI KPPKQQQTTE IHSDKLSRFT TSAEKEAKLV YSNSSSGPTA TLQKIPNTHL SSVTTSDLSP GPCHHSSLSQ IPSAIPSMPH QPTILLNTVS ASASPCLHPG AQNIPSPTGL PRCRSGSHTI GPFSSFQSAA HIYSQKLSRP SSAKAGSCYL NKHHSGIAKT QKEGEDASLY SKRYNQSMVT AELQRLAEKQ AARQYSPSSH INLLTQQVTN LNLATGIINR SSASAPPTLR PIISPSGPTW STQSDPQAPE NHSSSPGSRS LQTGGFAWEG EVENNVYSQA TGVVPQHKYH PTAGSYQLQF ALQQLEQQKL QSRQLLDQSR ARHQAIFGSQ TLPNSNLWTM NNGAGCRISS ATASGQKPTT LPQKVVPPPS SCASLVPKPP PNHEQVLRRA TSQKASKGSS AEGQLNGLQS SLNPAAFVPI TSSTDPAHTK I Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: TTLL5 (Tubulin tyrosine Ligase-Like Family, Member 5 (TTLL5))

Alternative Name: TTLL5

Background: Tubulin polyglutamylase TTLL5 (EC 6.3.2.-) (SRC1 and TIF2-associated modulatory protein) (STAMP protein) (Tubulin--tyrosine ligase-like protein 5),FUNCTION: Polyglutamylase which modifies tubulin, generating polyglutamate side chains on the gamma-carboxyl group of specific glutamate residues within the C-terminal tail of tubulin. Preferentially mediates ATP-dependent initiation step of the polyglutamylation reaction over the elongation step. Preferentially modifies the alpha-tubulin tail over a beta-tail (By similarity). Required for CCSAP localization to both polyglutamylated spindle and cilia microtubules (PubMed:22493317). Increases the effects of transcriptional coactivator NCOA2/TIF2 in glucocorticoid receptor-mediated repression and induction and in androgen receptor-mediated induction (PubMed:17116691). {ECO:0000250|UniProtKB:Q8CHB8, ECO:0000269|PubMed:17116691, ECO:0000269|PubMed:22493317}.

Molecular Weight: 143.6 kDa

UniProt: Q6EMB2

AlphaFold: Q6EMB2