IRG1 Protein (AA 1-488) (His tag)

Catalog No. ABIN7556176

This Recombinant IRG1 protein is produced in HEK-293 Cells.

Quick Overview for IRG1 Protein (AA 1-488) (His tag) (ABIN7556176)

Target: IRG1 (Immunoresponsive Gene 1 (IRG1))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-488

Purification tag / Conjugate: This IRG1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Acod1 Protein expressed in mammalian cells.

Sequence: MMLKSVTESF AGMIHGLKVN HLTDGIIRRS KRMILDSLGV GFLGTGTEVF HKVTQYSKIY SSNTSSTVWG RPDFRLPPTY AAFVNGVAVH SMDFDDTWHP ATHPSGAVLP VLTALSEALP QIPKFSGLDL LLAFNVGIEV QGRLMHFSKE AKDIPKRFHP PSVVGTLGSA AAASKFLGLS LTKCREALAI AVSHAGAPIA NAATQTKPLH IGNAAKHGME ATFLAMLGLQ GNKQILDLGS GFGAFYANYS PEDLPSLDSH IWLLDQQDVA FKSFPAHLAT HWVADAAAAV RKHLVTPERA LFPADHIERI VLRIPDVQYV NRPFPDSEHE ARHSFQYVAC ASLLDGSITV PSFHSQQVNR PQVRELLKKV KLEHPPDNPP SFDTLYCEIS ITLKDGTTFT ERSDTFYGHW RKPLSQEDLR NKFRANASKM LCRDTVESLI TVVEKLEDLE DCSVLTRLLK GPSVQDEASK LSSMSSFDHT TLPRFTNI Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: IRG1 (Immunoresponsive Gene 1 (IRG1))

Alternative Name: Acod1

Background: Cis-aconitate decarboxylase (CAD) (EC 4.1.1.6) (Aconitate decarboxylase) (Aconitate decarboxylase 1) (Cis-aconitic acid decarboxylase) (Immune-responsive gene 1 protein),FUNCTION: Cis-aconitate decarboxylase that catalyzes production of itaconate and is involved in the inhibition of the inflammatory response (PubMed:23609450, PubMed:23610393, PubMed:30635240, PubMed:31548418). Acts as a negative regulator of the Toll-like receptors (TLRs)-mediated inflammatory innate response by stimulating the tumor necrosis factor alpha-induced protein TNFAIP3 expression via reactive oxygen species (ROS) in LPS-tolerized macrophages (PubMed:23609450). Involved in antimicrobial response of innate immune cells, ACOD1-mediated itaconic acid production contributes to the antimicrobial activity of macrophages by generating itaconate, leading to alkylation of proteins, such as TFEB (PubMed:23610393, PubMed:35662396). Involved in antiviral response following infection by flavivirus in neurons: ACOD1-mediated itaconate production inhibits the activity of succinate dehydrogenase, generating a metabolic state in neurons that suppresses replication of viral genomes (PubMed:30635240). Plays a role in the embryo implantation (PubMed:14500577). {ECO:0000269|PubMed:14500577, ECO:0000269|PubMed:23609450, ECO:0000269|PubMed:23610393, ECO:0000269|PubMed:30635240, ECO:0000269|PubMed:31548418, ECO:0000269|PubMed:35662396}.

Molecular Weight: 53.8 kDa

UniProt: P54987

AlphaFold: P54987