ORC5 Protein (AA 1-435) (His tag)

Catalog No. ABIN7556293

This Recombinant ORC5 protein is produced in HEK-293 Cells.

Quick Overview for ORC5 Protein (AA 1-435) (His tag) (ABIN7556293)

Target: ORC5 (Origin Recognition Complex, Subunit 5 (ORC5))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-435

Purification tag / Conjugate: This ORC5 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Orc5 Protein expressed in mammalian cells.

Sequence: MSHLESMVLC REAQVSTLQS LFGERHHFSF PSIFIYGHTA SGKTYVTQTL LKTLELPHAF VNCVECFTSR LLLEQILNKL SHLNSSDAGC STEMTCETFN DFVQLFKQVT SAEHLKDQTV YIVLDKAEYL RDMEANLLPG LLRLQELTDR NVTVIFLSEI IWEKFRPNTG CFEPFVLYFP DYSIGNLQKI LSHDHPPEYS ADFYAAYINI LLGVFYTVCR DLKELRHLAV LNFPKYCEPV VKGEAGERDT RKLWRNIEPH LKKAMQTVYL REISSSQWEK LQKDNTDPGQ LKGLSAYTHM ELPYYSKFIL IAAYLASYNP ARTDKRFFLK HHGKIKKTNF LKKHEKTSNH LLGPKPFPLD RLLAILYSIV DSRVAPTANI FSQITSLVTL QLLTIVGHED QLNGPKYKCT VSLDFIRAIA RMVNFDIIKY LYDFL Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ORC5 (Origin Recognition Complex, Subunit 5 (ORC5))

Alternative Name: Orc5

Background: Origin recognition complex subunit 5,FUNCTION: Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. {ECO:0000250|UniProtKB:O43913}.

Molecular Weight: 50.2 kDa

UniProt: Q9WUV0

AlphaFold: Q9WUV0

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA