AP4B1 Protein (AA 1-738) (His tag)

Catalog No. ABIN7556306

This Recombinant AP4B1 protein is expressed in HEK-293 Cells.

Quick Overview for AP4B1 Protein (AA 1-738) (His tag) (ABIN7556306)

Target: AP4B1 (Adaptor-Related Protein Complex 4, beta 1 Subunit (AP4B1))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-738

Purification tag / Conjugate: This AP4B1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Ap4b1 Protein expressed in mammalian cells.

Sequence: MPYLGSEDVV KELKKALCNP HIQADRLRYR NVIQRVIRHM TQGLDMSDVF MEMVKASATV DIVQKKLVYL YMGTYAPLKP DLALLAINTL CKDCSDPNPM VRGLALRSMC SLRMPGVQEY IQQPVVNGLR DKASYVRRVA VLGCAKMHNL HGDSEVDGAL VNELYSLLRD QDPIVVVNCL RSLEEILKQE GGVVINKPIA HHLLNRMSKL DQWGQAEVLN FLLRYQPRSE EELFDILNLL DSYLKSSSTG VVMGATKLFL ILAKKFPHVQ TDVLVRVKGP LLAACSSESR ELCFAALCHV RQVLHSLPGH FSSHYKKFFC SYSEPHYIKL QKVEVLCELV NDENVQQVLE ELRGYCTDVA ADFAQAAIFA IGSIAKTYTD QCVQILTELL GLRQEHITTV VVQTFRDLVW LCPQCTEAVC QALPGCEENI QDSEGKQALI WLLGVHGEKI PNAPYVLEDF VDNVKSETFP AVKMELLTAL MRLVLSRPAE CQDMLGRLLH YCIEEEKDMA VRDRGLFYYR LLLVGIDKVK QILCSPKSDP SLGLLEDQPE RPVNSWASDF NTLAPVYGRA HWATISKCQQ VERHRLELPH NASFATSGHL ISEENKEGAQ EPDSDTLMLV PNLQLTAEYF EKTWLSLRVS YQQVFPWQGE VQPDTLQMAL KVVNIQTIAM SRAGAQPWKA YLSAQDDTGG LFLAELLLKP ENSEMQISVK QSKARTESLH GFVSVLETVI GTVGDIKS Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: AP4B1 (Adaptor-Related Protein Complex 4, beta 1 Subunit (AP4B1))

Alternative Name: Ap4b1

Background: AP-4 complex subunit beta-1 (AP-4 adaptor complex subunit beta) (Adaptor-related protein complex 4 subunit beta-1) (Beta subunit of AP-4) (Beta4-adaptin),FUNCTION: Component of the adaptor protein complex 4 (AP-4). Adaptor protein complexes are vesicle coat components involved both in vesicle formation and cargo selection. They control the vesicular transport of proteins in different trafficking pathways. AP-4 forms a non clathrin-associated coat on vesicles departing the trans-Golgi network (TGN) and may be involved in the targeting of proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system (By similarity). It is also involved in protein sorting to the basolateral membrane in epithelial cells and the proper asymmetric localization of somatodendritic proteins in neurons (PubMed:18341993). AP-4 is involved in the recognition and binding of tyrosine-based sorting signals found in the cytoplasmic part of cargos, but may also recognize other types of sorting signal (By similarity). {ECO:0000250|UniProtKB:Q9Y6B7, ECO:0000269|PubMed:18341993}.

Molecular Weight: 83.0 kDa

UniProt: Q9WV76

AlphaFold: Q9WV76