Ancient Ubiquitous Protein 1 Protein (Aup1) (AA 1-410) (His tag)

Catalog No. ABIN7556688

Recombinant Ancient Ubiquitous Protein 1 protein expressed in HEK-293 Cells.

Quick Overview for Ancient Ubiquitous Protein 1 Protein (Aup1) (AA 1-410) (His tag) (ABIN7556688)

Target: Ancient Ubiquitous Protein 1 (Aup1)

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-410

Purification tag / Conjugate: This Ancient Ubiquitous Protein 1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Aup1 Protein expressed in mammalian cells.

Sequence: MEPPPAPGPE RLFDSHRLPS DGFLLLALLL YAPVGLCLLV LRLFLGLHVF LVSCALPDSV LRRFVVRTMC AVLGLVARQE DSGLRDHRVR VLISNHVTPF DHNIVNLLTT CSTPLLNSPP SFVCWSRGFM EMDRRVELVE SLKKFCASTR LPPTPLLLFP EEEATNGREG LLRFSSWPFS IQDVVQPLTL QVQRPLVSVT VSDASWVSEL LWSLFVPFTV YQVRWLHPIR RQLGEESEEF ALRVQQLVAK ELGQIGTRLT PADKAEHMKR QRHPRLRPQS VQSSFPSPPS PSSDVQLTTL AHRVKEVLPH VPLNVIQRDL ARTGCVDLTI TNLLEGAVAF MPEDVTEGSQ SPPAPSAPKF PSSGLATPQP TALTFAKSSW ARQESLQERK QALYEYARRR FRERQAQEAE Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Ancient Ubiquitous Protein 1 (Aup1)

Alternative Name: Aup1

Background: Lipid droplet-regulating VLDL assembly factor AUP1 (Ancient ubiquitous protein 1),FUNCTION: Plays a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome (By similarity). Plays a role in lipid droplet formation (By similarity). Induces lipid droplet clustering (By similarity). Recruits ubiquitin-conjugating enzyme UBE2G2 to lipid droplets which facilitates its interaction with ubiquitin ligases AMFR/gp78 and RNF139/TRC8, leading to sterol-induced ubiquitination of HMGCR and its subsequent proteasomal degradation (By similarity). Also required for the degradation of INSIG1, SREBF1 and SREBF2 (By similarity). Plays a role in regulating assembly and secretion of very low density lipoprotein particles and stability of apolipoprotein APOB (By similarity). {ECO:0000250|UniProtKB:Q9Y679}.

Molecular Weight: 46.1 kDa

UniProt: P70295

AlphaFold: P70295