HSD17B3 Protein (AA 1-305) (His tag)

Catalog No. ABIN7556711

This Recombinant HSD17B3 protein is expressed in HEK-293 Cells.

Quick Overview for HSD17B3 Protein (AA 1-305) (His tag) (ABIN7556711)

Target: HSD17B3 (Hydroxysteroid (17-Beta) Dehydrogenase 3 (HSD17B3))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-305

Purification tag / Conjugate: This HSD17B3 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Hsd17b3 Protein expressed in mammalian cells.

Sequence: MEKLFIAAGL FVGLVCLVKC MRFSQHLFLR FCKALPSSFL RSMGQWAVIT GAGDGIGKAY SFELARHGLN VVLISRTLEK LQTIAEEIER TTGSCVKIVQ ADFTREDIYD HIKEHLEGLE IGILVNNVGM LPSFFPSHFL STSGESQNLI HCNITSVVKM TQLVLKHMES RRKGLILNIS SGAALRPWPL YSLYSASKAF VYTFSKALSV EYRDKGIIIQ VLTPYSISTP MTKYLNNKMT KTADEFVKES LKYVTIGAES CGCLAHEIIA IILNRIPSRI FYSSTAQRFL LTRYSDYLKR NISNR Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: HSD17B3 (Hydroxysteroid (17-Beta) Dehydrogenase 3 (HSD17B3))

Alternative Name: Hsd17b3

Background: 17-beta-hydroxysteroid dehydrogenase type 3 (17-beta-HSD 3) (Estradiol 17-beta-dehydrogenase 2) (EC 1.1.1.62) (Testicular 17-beta-hydroxysteroid dehydrogenase) (Testosterone 17-beta-dehydrogenase 3) (EC 1.1.1.64),FUNCTION: Catalyzes the conversion of 17-oxosteroids to 17beta-hydroxysteroids (PubMed:32557858). Favors the reduction of androstenedione to testosterone (PubMed:32557858). Testosterone is the key androgen driving male development and function (PubMed:32190925, PubMed:32557858). Uses NADPH while the two other EDH17B enzymes use NADH (By similarity). Androgens such as epiandrosterone, dehydroepiandrosterone, androsterone and androstanedione are accepted as substrates and reduced at C-17. Can reduce 11-ketoandrostenedione as well as 11beta-hydroxyandrostenedione at C-17 to the respective testosterone forms (By similarity). Plays a role in the rate-limiting-step for the maximum level of testosterone production by the testis but does not affect basal testosterone production (PubMed:32190925, PubMed:32557858). {ECO:0000250|UniProtKB:P37058, ECO:0000269|PubMed:32190925, ECO:0000269|PubMed:32557858, ECO:0000303|PubMed:32190925, ECO:0000303|PubMed:32557858}.

Molecular Weight: 34.3 kDa

UniProt: P70385

AlphaFold: P70385

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis