Gasdermin C2 Protein (GSDMC2) (AA 1-480) (His tag)

Catalog No. ABIN7557002

This Recombinant Gasdermin C2 protein is expressed in HEK-293 Cells.

Quick Overview for Gasdermin C2 Protein (GSDMC2) (AA 1-480) (His tag) (ABIN7557002)

Target: Gasdermin C2 (GSDMC2)

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-480

Purification tag / Conjugate: This Gasdermin C2 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Gsdmc2 Protein expressed in mammalian cells.

Sequence: MGYSFDRASK DVVKKLQGRD LRPVECLSDA TKFRLFHILQ ETPRSGWETE DIPVGFTLLD LLEPNFPVPE PEVSAPKPFI HVQSTDLEAN LNVADIARGG VGYVGYGGYN IEVQSTSIPN PKLEILQNRK LLDNLPTFMK FCRMERKNLY VVTEAYEVSK DTMLTGLSSV NLSVKGFFKQ LFKVRGKAGR SEKYSIPIPK GSVLAYKKQQ LVIENNTCVI LPSATKKKMT FPGTPKYASA SEPTEIYRTE LQGLWINDIV PIGRIQEPAH LDFMCLQNEV YKQTEQLAEL SKGVQEVVLS SILSMLYEGD RKVLYDLMNM LELNQLGHMD GPGGKILDEL RKDSSNPCVD LKDLILYLLQ ALMVLSDSQL NLLAQSVEMG ILPHQVELVK SILQPNFKYP WNIPFTLQPQ LLAPLQGEGL AITYELLEEC GLKMELNNPR STWDLEAKMP LSALYGSLSF LQQLRKANSS SKPSLRPGYI Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Gasdermin C2 (GSDMC2)

Alternative Name: Gsdmc2

Background: Gasdermin-C2 [Cleaved into: Gasdermin-C2, N-terminal (GSDMC2-NT), Gasdermin-C2, C-terminal (GSDMC2-CT)],FUNCTION: [Gasdermin-C2]: This form constitutes the precursor of the pore-forming protein: upon cleavage, the released N-terminal moiety (Gasdermin-C2, N-terminal) binds to membranes and forms pores, triggering pyroptosis. {ECO:0000269|PubMed:34290141}., FUNCTION: [Gasdermin-C2, N-terminal]: Pore-forming protein that causes membrane permeabilization and pyroptosis in response to type-2 immunity (PubMed:34290141). Produced by the cleavage of gasdermin-D in response to type-2 immunity following worm infection (PubMed:34290141). After cleavage, moves to the plasma membrane where it strongly binds to membrane inner leaflet lipids (By similarity). Homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, triggering pyroptosis and lytic cell death in enterocytes (PubMed:34290141). {ECO:0000250|UniProtKB:Q9BYG8, ECO:0000269|PubMed:34290141}.

Molecular Weight: 54.0 kDa

UniProt: Q2KHK6

AlphaFold: Q2KHK6