METTL4 Protein (AA 1-471) (His tag)

Catalog No. ABIN7557112

This Recombinant METTL4 protein is produced in HEK-293 Cells.

Quick Overview for METTL4 Protein (AA 1-471) (His tag) (ABIN7557112)

Target: METTL4 (Methyltransferase Like 4 (METTL4))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-471

Purification tag / Conjugate: This METTL4 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Mettl4 Protein expressed in mammalian cells.

Sequence: MSVVHHLPPG WLLDHLSFIN KVNYQLCQHQ ESFCSKNNPT SSVYMDSLQL DPGSPFGAPA MCFAPDFTTV SGNDDEGSCE VITEKYVFRS ELFNVTKPYI VPAVHKERQQ SNKNENLVTD YKQEVSVSVG KKRKRCIAFN QGELDAMEYH TKIRELILDG SSKLIQEGLR SGFLYPLVEK QDGSSGCITL PLDACNLSEL CEMAKHLPSL NEMELQTLQL MGDDVSVIEL DLSSQIIENN SSFSKMITLM GQKYLLPPQS SFLLSDISCM QPLLNCGKTF DAIVIDPPWE NKSVKRSNRY SSLSPQQIKR MPIPKLAAAD CLIVTWVTNR QKHLCFVKEE LYPSWSVEVV AEWYWVKITN SGEFVFPLDS PHKKPYECLV LGRVKEKTPL ALRNPDVRIP PVPDQKLIVS VPCVLHSHKP PLTEVLRDYI KPGGQCLELF ARNLQPGWMS WGNEVLKFQH MDYFIALESG C Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: METTL4 (Methyltransferase Like 4 (METTL4))

Alternative Name: Mettl4

Background: N(6)-adenine-specific methyltransferase METTL4 (Methyltransferase-like protein 4) (N(6)-adenine-specific DNA methyltransferase METTL4) (EC 2.1.1.72) (snRNA (2'-O-methyladenosine-N(6)-)-methyltransferase METTL4) (EC 2.1.1.-),FUNCTION: N(6)-adenine-specific methyltransferase that can methylate both RNAs and DNA (PubMed:30982744). Acts as a N(6)-adenine-specific RNA methyltransferase by catalyzing formation of N6,2'-O-dimethyladenosine (m6A(m)) on internal positions of U2 small nuclear RNA (snRNA): methylates the 6th position of adenine residues with a pre-deposited 2'-O-methylation (By similarity). Internal m6A(m) methylation of snRNAs regulates RNA splicing (By similarity). Also able to act as a N(6)-adenine-specific DNA methyltransferase by mediating methylation of DNA on the 6th position of adenine (N(6)-methyladenosine) (PubMed:30982744). The existence of N(6)-methyladenosine (m6A) on DNA is however unclear in mammals, and additional evidences are required to confirm the role of the N(6)-adenine-specific DNA methyltransferase activity of METTL4 in vivo (By similarity). Acts as a regulator of mitochondrial transcript levels and mitochondrial DNA (mtDNA) copy number by mediating mtDNA N(6)-methylation: m6A on mtDNA reduces transcription by repressing TFAM DNA-binding and bending (By similarity). N(6)-methyladenosine deposition by METTL4 regulates Polycomb silencing by triggering ubiquitination and degradation of sensor proteins ASXL1 and MPND, leading to inactivation of the PR-DUB complex and subsequent preservation of Polycomb silencing (PubMed:30982744). {ECO:0000250|UniProtKB:Q8N3J2, ECO:0000269|PubMed:30982744}.

Molecular Weight: 53.3 kDa

UniProt: Q3U034

AlphaFold: Q3U034