SMCR8 Protein (AA 1-935) (His tag)

Catalog No. ABIN7557228

Recombinant SMCR8 protein expressed in HEK-293 Cells.

Quick Overview for SMCR8 Protein (AA 1-935) (His tag) (ABIN7557228)

Target: SMCR8 (Smith-Magenis Syndrome Chromosome Region, Candidate 8 (SMCR8))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-935

Purification tag / Conjugate: This SMCR8 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Smcr8 Protein expressed in mammalian cells.

Sequence: MISAPDVVAF TKEDEYEEEP YNEPALPEEY SVPLFPYASQ GANPWSKLSG AKFSRDFILI SEFSEQVGPQ PLLTIPNDTK VFGTFDLNYF SLRIMSVDYQ ASFVGHPPGS AYPKLNFVED SKVVLGDSKE GAFAYVHHLT LYDLEARGFV RPFCMAYISA DQHKIMQQFQ ELSAEFSKAS ECLKMGNRKA FAGELEKKLK DLDYTRTVLH TETEIQKKAN DKGFYSSQAI EKANELANVE KSIIEHQDLL RQIRSYPRQK TKIPDLQPGD TEHTQDQADQ VSTTSNPEES ANADLYTCRP AYTPKLIKAK STKCFDKKLK TLEELCDTEY FTQTLAQLSH IEHMFRGDLC YLLTSQIDRV LRKQQPITNF LFEDFVEVDD RMEKQENVPS QPSQDRLPPK PVEECPIPKV LISVGSYKSS VESVLIKMEQ ELGDEEYTGV EATEARSFDP QENLDYLDMD MKGSISSGES IEVLGTEKSA SVLSKSDSQA SLTVPLSPHV VRSKAVSHRT ISEDSIEVLS TCPSEALIPD DFKASYPSAI NEEEAYADNE GAIHFQASAG SPEPDETQEG NLENIPSQID SSCCIGKESE GHLVPLPTPA YTLSDEDSVV SIPPQRYIQK DQGLHVDFGV ENTDPSPRDN SCEMFPAYEL DPSCLLASRD VSKMSLDNYS DTTSYMGSAA STSSDRIPSA PPAGLSSERH KKRAGQNALK FIRQYPFAHP AIYSLLSGRT LVVLGEDETI VRKLVTALSI FVPNYGCYAK PVKHWISSPL HIMDFQKWKL IGLQRVASPA NVGTLHTLSR YSRYTSILDL DSKTLRCPLY RGTLVPRLAD HRTQIKRGST YYLHVQSMLT QLCSKAFLYT FCHHLHLPAH SEETQEAVAS RQTSFLKLNL GLVNEDIRVV QYLAELLKLH YMQESPGTTH PLLRFDYVPS FLYKI Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SMCR8 (Smith-Magenis Syndrome Chromosome Region, Candidate 8 (SMCR8))

Alternative Name: Smcr8

Background: Guanine nucleotide exchange protein SMCR8 (Smith-Magenis syndrome chromosomal region candidate gene 8 protein homolog),FUNCTION: Component of the C9orf72-SMCR8 complex, a complex that has guanine nucleotide exchange factor (GEF) activity and regulates autophagy (PubMed:27617292). In the complex, C9orf72 and SMCR8 probably constitute the catalytic subunits that promote the exchange of GDP to GTP, converting inactive GDP-bound RAB8A and RAB39B into their active GTP-bound form, thereby promoting autophagosome maturation (By similarity). The C9orf72-SMCR8 complex also acts as a negative regulator of autophagy initiation by interacting with the ULK1/ATG1 kinase complex and inhibiting its protein kinase activity (PubMed:27617292). As part of the C9orf72-SMCR8 complex, stimulates RAB8A and RAB11A GTPase activity in vitro (By similarity). Acts as a regulator of mTORC1 signaling by promoting phosphorylation of mTORC1 substrates (By similarity). In addition to its activity in the cytoplasm within the C9orf72-SMCR8 complex, SMCR8 also localizes in the nucleus, where it associates with chromatin and negatively regulates expression of suppresses ULK1 and WIPI2 genes (By similarity). {ECO:0000250|UniProtKB:Q8TEV9, ECO:0000269|PubMed:27617292}.

Molecular Weight: 105.0 kDa

UniProt: Q3UMB5

AlphaFold: Q3UMB5