Ube2fb (AA 1-185) protein (His tag)

Catalog No. ABIN7557299

This Recombinant protein is expressed in HEK-293 Cells.

Quick Overview for Ube2fb (AA 1-185) protein (His tag) (ABIN7557299)

Target: Ube2fb

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-185

Purification tag / Conjugate: His tag

Purpose: Predefined custom protein recombinant Ube2fb Protein expressed in mammalian cells.

Sequence: MLTLASKLKW DEDLKGSQTS VSTSDSTRRF SMRDKLLVTE VAELEANLPW TCKVHFPDPN KLHCFQLTVS PDEGYYQGGN FQFEIEVPDA YNMVPPKVKC LTKIWHPNIT ETGEICLSLL REHSADGTGW APTRTLKDVV WGLNSLFTDL LNFYDPLNIE AAEHHLQDKE DFRDKVDEYI RRYAR Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Ube2fb

Background: NEDD8-conjugating enzyme UBE2F-B (EC 2.3.2.34) (NEDD8 carrier protein UBE2F-B) (NEDD8 protein ligase UBE2F-B) (RING-type E3 NEDD8 transferase UBE2F-B) (Ubiquitin-conjugating enzyme E2 F-B),FUNCTION: Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5. {ECO:0000250}.

Molecular Weight: 21.4 kDa

UniProt: Q3UWQ3

AlphaFold: Q3UWQ3