Sh2d1b2 Protein (AA 1-132) (His tag)

Catalog No. ABIN7557363

Recombinant Sh2d1b2 protein expressed in HEK-293 Cells.

Quick Overview for Sh2d1b2 Protein (AA 1-132) (His tag) (ABIN7557363)

Target: Sh2d1b2 (SH2D1B2) (SH2 Domain Protein 1B2 (SH2D1B2))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-132

Purification tag / Conjugate: This Sh2d1b2 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Sh2d1b2 Protein expressed in mammalian cells.

Sequence: MDLPYYHGCL TKRECEALLL KGGVDGNFLI RDSESVPGAL CLCVSFKKLV YNYRIFREKN GYYRIETEPS TPKTIFPNLE ELISKFKTPG QGMVVHLSNP IMRSGFCPGA RRLNLEANVY ENTDEEYVDV LP Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Sh2d1b2 (SH2D1B2) (SH2 Domain Protein 1B2 (SH2D1B2))

Alternative Name: Sh2d1b2

Background: SH2 domain-containing protein 1B2 (EAT-2-related transducer) (ERT) (EAT-2B),FUNCTION: Cytoplasmic adapter regulating receptors of the signaling lymphocytic activation molecule (SLAM) family. In SLAM signaling may cooperate with Sh2d1a/SAP. Plays a role in regulation of effector functions of natural killer (NK) cells by controlling signal transduction through Cd244/2b4. However, conflicting results are reported which may reflect the use of different strain backgrounds. Proposed to act as an inhibitor of Cd244-mediated NK cell function including cytotoxicity and IFN-gamma production, the latter found also by triggering Klra4 and Klrk1 next to Cd244 (PubMed:16127454). Seems to positively regulate Cd244- and Cd84-dependent NK cell functions implicating Cd244-mediated phosphorylation of Vav1 (PubMed:20962259). {ECO:0000269|PubMed:16127454, ECO:0000269|PubMed:20962259}.

Molecular Weight: 15.0 kDa

UniProt: Q45HK4

AlphaFold: Q45HK4