G2E3 Protein (AA 1-716) (His tag)

Catalog No. ABIN7557515

This Recombinant G2E3 protein is expressed in HEK-293 Cells.

Quick Overview for G2E3 Protein (AA 1-716) (His tag) (ABIN7557515)

Target: G2E3 (G2/M-Phase Specific E3 Ubiquitin Protein Ligase (G2E3))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-716

Purification tag / Conjugate: This G2E3 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant G2e3 Protein expressed in mammalian cells.

Sequence: MNENKPDNSQ SLACVFCRKN DDCPNKYGEK KTYEKWNFSV HYYCLLMSSG IWQRGKEEEG VYGFLIEDIR KEVQRASKLK CTVCKKNGAS IGCVVPTCKR SYHLPCGLQK ECIFQFTDNF ASFCWKHRPV QAITSNKYSS SLPCTICLEF VEPIPTYNIL QSPCCKNAWF HRDCLQVQAI NAGVFFFRCT LCNNTDIFQK EMLRMGIHIP EKDASWELEE NAYQELLQSH DRCDIRRCHC KKGRDYNEPN SKWEVKRCQS CGSSGTHLAC SSLQSWEQNW ECLDCRRITY TSDFQKAPKH PLANSTNVTV TDCLLEESSS KLPRQSTVAQ HKELLRQGSK FRRDISTILI ELGFQIKKKT KTLYINKANV WRSALEQFQS QKFNPSCSID VVYVNGNEVG SQHLGSKQEF LSHLMHHLEN SSVFEGSLAK NLSLNSQAVK ENLYYEVGKM LAISLVHGGP SPGFFSETLF NCLAYGPENT LPTLDDVSDI DVAQIIIKID SATDLNILNS VISQHYNYLE VSGCLRLTTS LSDKFMLVKD ILFYHVINRV KAPFESFKQG LKTLGVLEKI QTYPEAFYKI LCHKPENLSA KNLSDLFTIH SVADVQTLRF WNSYLKAIED GKSATTMEDI LIFATGCSSV PPTGFKPSLS VECLHVDFPV ADKYRNHLVL PATNTYEEFQ ENMDFTIRDT LRLEKEERSH ILPRTLNVSS NEEMLI Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: G2E3 (G2/M-Phase Specific E3 Ubiquitin Protein Ligase (G2E3))

Alternative Name: G2e3

Background: G2/M phase-specific E3 ubiquitin-protein ligase (EC 2.3.2.26) (G2/M phase-specific HECT-type E3 ubiquitin transferase),FUNCTION: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (By similarity). Required for prevention of apoptotic death in early embryogenesis. {ECO:0000250|UniProtKB:Q7L622, ECO:0000269|PubMed:18511420}.

Molecular Weight: 81.8 kDa

UniProt: Q5RJY2

AlphaFold: Q5RJY2