NR1H2 Protein (AA 1-446) (His tag)

Catalog No. ABIN7557585

Recombinant NR1H2 protein expressed in HEK-293 Cells.

Quick Overview for NR1H2 Protein (AA 1-446) (His tag) (ABIN7557585)

Target: NR1H2 (Nuclear Receptor Subfamily 1, Group H, Member 2 (NR1H2))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-446

Purification tag / Conjugate: This NR1H2 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Nr1h2 Protein expressed in mammalian cells.

Sequence: MSSPTSSLDT PVPGNGSPQP STSATSPTIK EEGQETDPPP GSEGSSSAYI VVILEPEDEP ERKRKKGPAP KMLGHELCRV CGDKASGFHY NVLSCEGCKG FFRRSVVHGG AGRYACRGSG TCQMDAFMRR KCQLCRLRKC KEAGMREQCV LSEEQIRKKR IQKQQQQQPP PPSEPAASSS GRPAASPGTS EASSQGSGEG EGIQLTAAQE LMIQQLVAAQ LQCNKRSFSD QPKVTPWPLG ADPQSRDARQ QRFAHFTELA IISVQEIVDF AKQVPGFLQL GREDQIALLK ASTIEIMLLE TARRYNHETE CITFLKDFTY SKDDFHRAGL QVEFINPIFE FSRAMRRLGL DDAEYALLIA INIFSADRPN VQEPSRVEAL QQPYVEALLS YTRIKRPQDQ LRFPRMLMKL VSLRTLSSVH SEQVFALRLQ DKKLPPLLSE IWDVHE Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: NR1H2 (Nuclear Receptor Subfamily 1, Group H, Member 2 (NR1H2))

Alternative Name: Nr1h2

Background: Oxysterols receptor LXR-beta (Liver X receptor beta) (Nuclear receptor subfamily 1 group H member 2) (Retinoid X receptor-interacting protein No.15) (Ubiquitously-expressed nuclear receptor),FUNCTION: Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity (PubMed:18055760, PubMed:19520913, PubMed:20427281). Binds preferentially to double-stranded oligonucleotide direct repeats having the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4) (PubMed:18055760, PubMed:19520913, PubMed:20427281). Regulates cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8, DLDLR and LRP8 (PubMed:18055760, PubMed:19520913, PubMed:20427281). Interplays functionally with RORA for the regulation of genes involved in liver metabolism (PubMed:18055760, PubMed:19520913, PubMed:20427281, PubMed:24206663, PubMed:28846071). Induces LPCAT3-dependent phospholipid remodeling in endoplasmic reticulum (ER) membranes of hepatocytes, driving SREBF1 processing and lipogenesis (PubMed:28846071, PubMed:25806685). Via LPCAT3, triggers the incorporation of arachidonate into phosphatidylcholines of ER membranes, increasing membrane dynamics and enabling triacylglycerols transfer to nascent very low-density lipoprotein (VLDL) particles (PubMed:25806685). Via LPCAT3 also counteracts lipid-induced ER stress response and inflammation, likely by modulating SRC kinase membrane compartmentalization and limiting the synthesis of lipid inflammatory mediators (PubMed:24206663). Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor: inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex (By similarity). {ECO:0000250|UniProtKB:P55055, ECO:0000269|PubMed:18055760, ECO:0000269|PubMed:19520913, ECO:0000269|PubMed:20427281, ECO:0000269|PubMed:24206663, ECO:0000269|PubMed:25806685, ECO:0000269|PubMed:28846071}.

Molecular Weight: 49.7 kDa

UniProt: Q60644

AlphaFold: Q60644

Pathways: Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Nuclear Hormone Receptor Binding