SLC25A47 Protein (AA 1-310) (His tag)

Catalog No. ABIN7557935

This Recombinant SLC25A47 protein is expressed in HEK-293 Cells.

Quick Overview for SLC25A47 Protein (AA 1-310) (His tag) (ABIN7557935)

Target: SLC25A47 (Solute Carrier Family 25, Member 47 (SLC25A47))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-310

Purification tag / Conjugate: This SLC25A47 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Slc25a47 Protein expressed in mammalian cells.

Sequence: MDFVAGAIGG VCGVAVGYPL DTVKVRIQTE AKYAGIWHCI RDTYRQERVW GFYRGLSLPV CTVSLVSSVS FGTYHHCLAH ICRFRYGSTD AKPTKADITL SGCASGLVRV FLTSPTEVAK VRLQTQTQAQ TQQRRSSASW TSGAPALCPT PTACLEPRPK YSGPLHCLVT VAREEGLRGL YKGSSALLLR EGHSFATYFL SYAMLCEWLT PAGHSQPDVL GVLVAGGCAG VLAWAVATPM DVIKSRLQAD GQGQHRYRGL LHCVVTSVRE EGPRVLFKGL ALNCCRAFPV NMVVFVAYEA VLRLTQSLLT Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SLC25A47 (Solute Carrier Family 25, Member 47 (SLC25A47))

Alternative Name: Slc25a47

Background: Solute carrier family 25 member 47 (Hepatocellular carcinoma down-regulated mitochondrial carrier homolog) (Mitochondrial NAD(+) transporter SLC25A47),FUNCTION: Mitochondrial NAD(+) transporter that acts as a 'metabolic gate' in hepatic lipogenesis. Provides NAD(+) substrate to mitochondrial SIRT3 deacetylase and enables its NAD(+)-dependent activities in mitochondrial energy metabolism. This triggers downstream activation of PRKAA1/AMPK-alpha signaling cascade that negatively regulates sterol regulatory element-binding protein (SREBP) transcriptional activities and ATP-consuming lipogenesis to restore cellular energy balance. May transport other mitochondrial metabolites having an aromatic nucleotide and phosphate groups, such as acetyl-CoA. Does not transport amino acids. The transport mechanism remains to be elucidated. {ECO:0000269|PubMed:36804859}.

Molecular Weight: 33.6 kDa

UniProt: Q6IS41

AlphaFold: Q6IS41