DCAF13 Protein (AA 1-445) (His tag)

Catalog No. ABIN7558025

This Recombinant DCAF13 protein is expressed in HEK-293 Cells.

Quick Overview for DCAF13 Protein (AA 1-445) (His tag) (ABIN7558025)

Target: DCAF13 (DDB1 and CUL4 Associated Factor 13 (DCAF13))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-445

Purification tag / Conjugate: This DCAF13 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Dcaf13 Protein expressed in mammalian cells.

Sequence: MKVKMLSRNP DNYVRETKLD IQRVPRNYDP TLHPFEVPRE YVRALNATKL ERVFAKPFLA SLDGHRDGVN CLAKHPKSLA SVLSGACDGE VKIWNLTKRK CIRTIQAHEG FVRGMCTRFC GTSFFTVGDD KTVKQWKMDG PGYGEEEEPL YTVLGKTVYT GIDHHWKDPV FATCGQQVDI WDEQRTSPVC SMNWGFDSIS SVKFNPVETF LLGSCASDRN IVLYDMRQAT PLKKVILEMR TNTICWNPME AFNFTAANED YNLYTFDMRA LDTPVMVHMD HVSAVLDVDY SPTGKEFVSA SFDKSIRIFP VDKSRSREVY HTKRMQHVMC VKWTSDSKYI MCGSDEMNIR LWKANASEKL GVLTSREKAA NDYNQKLKEK FQYHPHVKRI ARHRHLPKSI YSQIQEQRVM KEARRRKEMN RRKHSKPGSV PIVSERKKHV VAVVK Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: DCAF13 (DDB1 and CUL4 Associated Factor 13 (DCAF13))

Alternative Name: Dcaf13

Background: DDB1- and CUL4-associated factor 13 (WD repeat and SOF domain-containing protein 1),FUNCTION: Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (By similarity). Participates in the 18S rRNA processing in growing oocytes, being essential for oocyte nonsurrounded nucleolus (NSN) to surrounded nucleolus (SN) transition (PubMed:30283081). {ECO:0000250|UniProtKB:Q9NV06, ECO:0000269|PubMed:30283081}., FUNCTION: Substrate-recognition component of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex that plays a key role in embryo preimplantation and is required for normal meiotic cycle progression in oocytes (PubMed:30111536, PubMed:31492966). Acts as a maternal factor that regulates oocyte and zygotic chromatin tightness during maternal to zygotic transition (PubMed:31000741). Also involved in the transformation of the endometrium into the decidua, known as decidualization, providing a solid foundation for implantation of blastocysts (PubMed:35932979). Recognizes the histone methyltransferases SUV39H1 and SUV39H2 and directs them to polyubiquitination and proteasomal degradation, which facilitates the H3K9me3 removal and early zygotic gene expression, essential steps for progressive genome reprogramming and the establishment of pluripotency during preimplantation embryonic development (PubMed:30111536). Supports the spindle assembly and chromosome condensation during oocyte meiotic division by targeting the polyubiquitination and degradation of PTEN, a lipid phosphatase that inhibits PI3K pathway as well as oocyte growth and maturation (PubMed:31492966). Targets PMP22 for polyubiquitination and proteasomal degradation (PubMed:35178836). {ECO:0000269|PubMed:30111536, ECO:0000269|PubMed:31000741, ECO:0000269|PubMed:31492966, ECO:0000269|PubMed:35178836, ECO:0000269|PubMed:35932979}.

Molecular Weight: 51.5 kDa

UniProt: Q6PAC3

AlphaFold: Q6PAC3