DOC2A Protein (AA 1-405) (His tag)

Catalog No. ABIN7558231

This Recombinant DOC2A protein is produced in HEK-293 Cells.

Quick Overview for DOC2A Protein (AA 1-405) (His tag) (ABIN7558231)

Target: DOC2A (Double C2-Like Domains, alpha (DOC2A))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-405

Purification tag / Conjugate: This DOC2A protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Doc2a Protein expressed in mammalian cells.

Sequence: MRGRRGDRMT INIQEHMAIN VCPGPIRPIR QISDYFPRRG PGPEGGGGGG GTGCGEAPAH LAPLALAPPA ALLGATTPDD GAEVDSYDSD DTTALGTLEF DLLYDQASCM LHCRILRAKG LKPMDFNGLA DPYVKLHLLP GACKANKLKT KTQRNTLNPV WNEELTYSGI TDDDITHKVL RISVCDEDKL SHNEFIGEIR VPLRRLKPSQ KKHFNICLER QVPLPSPSSM SAALRGISCY LKELEQAEQG PGLLEERGRI LLSLSYSSRR HGLLVGIVRC AHLAAMDVNG YSDPYVKTYL RPDVDKKSKH KTCVKKKTLN PEFNEEFFYE IELSTLATKT LEVTVWDYDI GKSNDFIGGV SLGPGARGEA QKHWNDCLHQ PDTALERWHT LTSELPPAAG AYPLA Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: DOC2A (Double C2-Like Domains, alpha (DOC2A))

Alternative Name: Doc2a

Background: Double C2-like domain-containing protein alpha (Doc2-alpha),FUNCTION: Calcium sensor which most probably regulates fusion of vesicles with membranes. Binds calcium and phospholipids. May be involved in calcium dependent neurotransmitter release through the interaction with UNC13A. May be involved in calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Regulates Ca(2+)-dependent secretory lysosome exocytosis in mast cells. {ECO:0000269|PubMed:18354201, ECO:0000269|PubMed:20150444}.

Molecular Weight: 44.6 kDa

UniProt: Q7TNF0

AlphaFold: Q7TNF0

Pathways: Synaptic Vesicle Exocytosis