TRMT2B Protein (AA 1-493) (His tag)

Catalog No. ABIN7558815

Recombinant TRMT2B protein expressed in HEK-293 Cells.

Quick Overview for TRMT2B Protein (AA 1-493) (His tag) (ABIN7558815)

Target: TRMT2B (tRNA Methyltransferase 2 Homolog B (TRMT2B))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-493

Purification tag / Conjugate: This TRMT2B protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Trmt2b Protein expressed in mammalian cells.

Sequence: MHNPRLFLSR AGFFSKPGLL PWDTGRPPDL SQSLLGRAYK GSFTSVIAKK HPSRQKYQKK QRHCRDDSWQ ERLADVVTPL WRLSYEAQLK VKFEAQKKLL QSLESHLKVL HGVSDTVAAH QSEGLRCLLH PIIPSPTTTG YRNKSTFSVY RSPDGNPKTV GYYLGTWKDG NVVCLPCNHL KNIPEKHSQV AQYYEVFLRQ SSVEPCLLFH EGGHWRELVV RTNRQGHTMA IVTFHPQGLS EEEVCVQKVT LKDFFTKGPG AICELTSLYF QESTMTRCSH QQSPYQLLFG EPHIFEDLLG LKIRISPDAF FQINTAGAEM LYRIIGELSG VNSESLLLDI CCGTGVIGLS VAQRASQVHG IELVEQAVED ARWTAAFNGV TNCEFHAGRA ETILPQLLKS QKDEKLTVAV VNPARAGLHY RVVRAIRNCR TIHTLVFVSC KPHGESTRNF IELCCPPNSA KQLLGDPFVL REAVPVDLFP HTPHCELVLL FTR Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: TRMT2B (tRNA Methyltransferase 2 Homolog B (TRMT2B))

Alternative Name: Trmt2b

Background: TRNA (uracil-5-)-methyltransferase homolog B (EC 2.1.1.35) (TRM2 homolog B) (rRNA (uracil-5-)-methyltransferase TRMT2B) (EC 2.1.1.-),FUNCTION: Mitochondrial S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the formation of 5-methyl-uridine in tRNAs and 12S rRNA (PubMed:31736397). Catalyzes the methylation of uridine at position 54 (m5U54) in all tRNAs (PubMed:31736397). Specifically methylates the uridine in position 425 of 12S rRNA (m5U425) (PubMed:31736397). Does not affect RNA stability or mitochondrial translation (By similarity). {ECO:0000250|UniProtKB:Q96GJ1, ECO:0000269|PubMed:31736397}.

Molecular Weight: 55.5 kDa

UniProt: Q8BQJ6

AlphaFold: Q8BQJ6