TRIM35 Protein (AA 1-501) (His tag)

Catalog No. ABIN7559060

This Recombinant TRIM35 protein is expressed in HEK-293 Cells.

Quick Overview for TRIM35 Protein (AA 1-501) (His tag) (ABIN7559060)

Target: TRIM35 (Tripartite Motif Containing 35 (TRIM35))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-501

Purification tag / Conjugate: This TRIM35 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Trim35 Protein expressed in mammalian cells.

Sequence: MEPGPSVSPG PSRSFKEELL CAVCYDPFRD AVTLRCGHNF CRRCVSGCWE VQTTPSCPVC KERAVPGELR TNHTLNNLVE TLLREEAEGA RWTGRRSPRP CRAHRAPLTL FCLEDKELLC CACQADARHQ EHRVQPIKDT AQDFRAKCKN MEHVLREKAK AFWALRRTYE AIAKHNEVQT TWLEGRIRDE FDKLRDFLRV EEQATLDAMK EESRKKHLQA EEKMKQLAEQ TEALAREIER LQMEMKEDDM TFLMKHKSRK RRLFCTVEPA PLQPGLLMDA CKYLESLQYR VWKKMLGSVE SVPFSLDPNT AAGWLKVADD LTSVINHGYR VQVENPERFS SAPCLLGSQV FSKGSHSWEV DVGGLPTWRV GVVRVQAHAQ AQAQADVGGE GHSHSCYHDT RSGFWYLCRT QGVDGDHCMT SDTATAPLVQ AMPRRLRVEL ECEEGELSFY DSERHCHLYT FHAHFGEVRP YFYLGASRGD GPPEPLRICH LRVSIKEELD I Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: TRIM35 (Tripartite Motif Containing 35 (TRIM35))

Alternative Name: Trim35

Background: E3 ubiquitin-protein ligase TRIM35 (EC 2.3.2.27) (Hemopoietic lineage switch protein 5) (Macrophage-derived apoptosis-inducing RBCC protein) (Protein MAIR) (Protein Nc8),FUNCTION: E3 ubiquitin-protein ligase that participates in multiple biological processes including cell death, glucose metabolism, and in particular, the innate immune response (By similarity) (PubMed:32562145). Mediates 'Lys-63'-linked polyubiquitination of TRAF3 thereby promoting type I interferon production via RIG-I signaling pathway. Can also catalyze 'Lys-48'-linked polyubiquitination and proteasomal degradation of viral proteins such as influenza virus PB2. Acts as a negative feedback regulator of TLR7- and TLR9-triggered signaling. Mechanistically, promotes the 'Lys-48'-linked ubiquitination of IRF7 and induces its degradation via a proteasome-dependent pathway. Reduces FGFR1-dependent tyrosine phosphorylation of PKM, inhibiting PKM-dependent lactate production, glucose metabolism, and cell growth (By similarity). {ECO:0000250|UniProtKB:Q9UPQ4, ECO:0000269|PubMed:32562145}.

Molecular Weight: 57.3 kDa

UniProt: Q8C006

AlphaFold: Q8C006