Paxx Protein (AA 1-205) (His tag)

Catalog No. ABIN7559451

Recombinant Paxx protein expressed in HEK-293 Cells.

Quick Overview for Paxx Protein (AA 1-205) (His tag) (ABIN7559451)

Target: Paxx (Non-homologous End Joining Factor (Paxx))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-205

Purification tag / Conjugate: This Paxx protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Paxx Protein expressed in mammalian cells.

Sequence: MAPPLLSLPL CILPPGSGSP RLVCYCERDS GGDGDRDDFN LYVTDAAELW STCFSPDSLA RLKARFGLSG AEDIHSRFRA ACQQQAVTVS LQEDRALITL SGDTPALAFD LSKVPSPEAA PRLQALTLSL AEHVCNLERR LAAAEETITS PKKNTQPAGT QFLPELDHQR GSSGPGVRRR CPGESLINPG FKSKKPAAGV DFDET Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Paxx (Non-homologous End Joining Factor (Paxx))

Alternative Name: Paxx

Background: Protein PAXX (Paralog of XRCC4 and XLF),FUNCTION: Non-essential DNA repair protein involved in DNA non-homologous end joining (NHEJ), participates in double-strand break (DSB) repair and V(D)J recombination (PubMed:27601299, PubMed:27798842, PubMed:27601633, PubMed:27830975, PubMed:28051062, PubMed:29077092). May act as a scaffold required for accumulation of the Ku heterodimer, composed of XRCC5/Ku80 and XRCC6/Ku70, at double-strand break sites and promote the assembly and/or stability of the NHEJ machinery (PubMed:28051062). Involved in NHEJ by promoting the ligation of blunt-ended DNA ends (By similarity). Together with NHEJ1/XLF, collaborates with DNA polymerase lambda (POLL) to promote joining of non-cohesive DNA ends (By similarity). Constitutes a non-essential component of classical NHEJ: has a complementary but distinct function with NHEJ1/XLF in DNA repair (PubMed:27601299, PubMed:27798842, PubMed:27830975, PubMed:28051062). {ECO:0000250|UniProtKB:Q9BUH6, ECO:0000269|PubMed:27601299, ECO:0000269|PubMed:27601633, ECO:0000269|PubMed:27798842, ECO:0000269|PubMed:27830975, ECO:0000269|PubMed:28051062, ECO:0000269|PubMed:29077092}.

Molecular Weight: 22.0 kDa

UniProt: Q8K0Y7

AlphaFold: Q8K0Y7